2,3,3',4',6-五氯联苯对青春期前雌性大鼠的肝脏酶诱导及急性内分泌效应

Hepatic enzyme induction and acute endocrine effects of 2,3,3',4',6-pentachlorobiphenyl in prepubertal female rats.

作者信息

Li M, Rhine C, Hansen L G

机构信息

Department of Geography, National Taiwan University, Taipei, Taiwan, Republic of China.

出版信息

Arch Environ Contam Toxicol. 1998 Jul;35(1):97-103. doi: 10.1007/s002449900355.

DOI:10.1007/s002449900355

PMID:9601926

Abstract

Polychlorinated biphenyls (PCBs) with the labile 2,3,6-substitution are important components of atmospheric and certain food chain exposures, but little is known about their biological activities. Chlorobiphenyl 110 (2,3,3',4',6-pentaCB) was investigated in weanling female rats dosed ip on days 21 and 22 and killed on day 23 of age. The initial preparation of CB 110 markedly induced 7-ethoxyresorufin O-dealkylase (EROD) activity and was found to be contaminated with coplanar 3,3',4,4',5-pentaCB (CB 126). The contaminated preparation (CB 110C) was purified with activated charcoal (CB 110P). The CB 110P induced pentoxyresorufin O-dealkylase (PROD), was weakly uterotropic and a modest depleter of serum thyroxine (T4). CB 110C caused increased liver weight, induced EROD, PROD, and UDP glucuronyl transferase activities and caused a greater depletion of serum T4; on the other hand, it suppressed the PROD induction and the uterotropic effect of CB 110P. Hepatic residues of CB 110 were a constant 2-3% of the dose while those of CB 126 (from CB 110C) increased with increasing dose to as much as 50% of the dose.

摘要

具有不稳定的2,3,6-取代基的多氯联苯（PCBs）是大气和某些食物链暴露的重要成分，但对其生物活性知之甚少。在出生后第21天和第22天对断乳雌性大鼠腹腔注射氯联苯110（2,3,3',4',6-五氯联苯），并在出生后第23天处死。最初制备的CB 110显著诱导7-乙氧基异吩恶唑酮O-脱烷基酶（EROD）活性，并且发现其被共平面的3,3',4,4',5-五氯联苯（CB 126）污染。用活性炭对受污染的制剂（CB 110C）进行纯化（CB 110P）。CB 110P诱导戊氧基异吩恶唑酮O-脱烷基酶（PROD），具有弱子宫促生长作用，并且是血清甲状腺素（T4）的适度消耗剂。CB 110C导致肝脏重量增加，诱导EROD、PROD和UDP葡萄糖醛酸基转移酶活性，并导致血清T4的更大消耗；另一方面，它抑制了CB 110P的PROD诱导和子宫促生长作用。CB 110的肝脏残留量为剂量的恒定2%-3%，而CB 126（来自CB 110C）的残留量随剂量增加而增加，高达剂量的50%。