Park P C, De Boni U
Department of Physiology, University of Toronto, Toronto, Ontario, M5S 1A8, Canada.
Chromosoma. 1998 May;107(2):87-95. doi: 10.1007/s004120050284.
Chromatin in interphase nuclei exhibits a topology that is associated with the transcriptional state of cells. We examined the spatial, intranuclear distribution of chromosome 17 and the ERBB-2 (HER2/neu) sequence thereon, relative to that of DNase-hypersensitive chromatin (DHC), in breast tumour cells exhibiting different levels of expression of ERBB-2. These sequences were specifically associated with the nuclear periphery, within a band of DHC. The remainder of the chromosome 17 mass showed no preferential position within the nucleus. The peripheral placement of ERBB-2 sequences is associated with a specific conformation of chromosome 17. We propose that the conformational organization of chromosome territories might represent a fundamental control mechanism in gene expression.
间期细胞核中的染色质呈现出一种与细胞转录状态相关的拓扑结构。我们研究了在具有不同ERBB-2表达水平的乳腺肿瘤细胞中，17号染色体及其上的ERBB-2（HER2/neu）序列相对于DNase超敏染色质（DHC）的空间核内分布。这些序列在DHC带内与核周特异性相关。17号染色体的其余部分在核内没有优先位置。ERBB-2序列的外周定位与17号染色体的特定构象有关。我们提出染色体区域的构象组织可能代表了基因表达中的一种基本控制机制。
