小鼠中Peg1/Mest印记分析。
Analysis of Peg1/Mest imprinting in the mouse.
作者信息
Reule M, Krause R, Hemberger M, Fundele R
机构信息
Institut für Biologie III der Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany.
出版信息
Dev Genes Evol. 1998 May;208(3):161-3. doi: 10.1007/s004270050168.
In the mouse, Peg1/Mest is widely expressed in mesoderm-derived tissues. In separate studies, it has been shown to be maternally imprinted, that is, only the paternally inherited allele is active in mice and in humans. Here, we provide evidence that Peg1/Mest is expressed at very low levels in all tissues of adult mice as assessed by RT-PCR. Moreover, by using species-specific polymorphisms in the Peg1/Mest sequence we can demonstrate that in adult mice the gene remains imprinted in all of these tissues.
在小鼠中，Peg1/Mest在中胚层来源的组织中广泛表达。在另外的研究中，已表明它是母系印记的，也就是说，在小鼠和人类中只有父系遗传的等位基因是活跃的。在这里，我们提供的证据表明，通过逆转录聚合酶链反应（RT-PCR）评估，Peg1/Mest在成年小鼠的所有组织中表达水平非常低。此外，通过利用Peg1/Mest序列中的物种特异性多态性，我们可以证明在成年小鼠中该基因在所有这些组织中仍然是印记的。
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