García-Ojeda E, Alonso J R, Briñón J G, Rogers J, Weruaga E, Arévalo R, Aijón J
Departamento de Biología Celular y Patología, Universidad de Salamanca, Spain.
Brain Res. 1998 Apr 6;789(1):101-10. doi: 10.1016/s0006-8993(98)00023-7.
Calretinin-immunoreactive structures in the anterior olfactory nucleus of the rat were studied using a polyclonal antibody, which does not cross-react with the highly homologous calcium-binding protein calbindin-D28k, and the avidin-biotin-peroxidase technique. Calretinin-immunopositive neurons were found in all regions of the anterior olfactory nucleus, with the highest number in the medial subdivision and dorsal transition area. The immunostained neurons, although morphologically heterogeneous, demonstrated typically small size. In addition to neuronal somata, calretinin-immunopositive fibres and terminals, some of them forming basket-like arrangements surrounding immunonegative neurons, were observed. Although calretinin and calbindin-D28k colocalize in several brain regions, and both proteins showed an extensive overlap in the anterior olfactory nucleus, immunostained semithin sections demonstrated that calretinin does not co-localize with calbindin-D28k in this nucleus.
使用一种不与高度同源的钙结合蛋白钙结合蛋白-D28k发生交叉反应的多克隆抗体和抗生物素蛋白-生物素-过氧化物酶技术，对大鼠前嗅核中钙视网膜蛋白免疫反应性结构进行了研究。在前嗅核的所有区域均发现了钙视网膜蛋白免疫阳性神经元，其中在内侧亚区和背侧过渡区数量最多。免疫染色的神经元虽然形态各异，但通常体积较小。除了神经元胞体，还观察到了钙视网膜蛋白免疫阳性纤维和终末，其中一些形成围绕免疫阴性神经元的篮状结构。尽管钙视网膜蛋白和钙结合蛋白-D28k在几个脑区共定位，且两种蛋白在前嗅核中显示出广泛重叠，但免疫染色的半薄切片表明，在该核中钙视网膜蛋白不与钙结合蛋白-D28k共定位。
