Kanazawa Y, Doi Y, Kudo H, Fujimoto S
J Electron Microsc (Tokyo). 1998;47(1):87-92. doi: 10.1093/oxfordjournals.jmicro.a023563.
In the rat hind limb bud aged between prenatal days 14 and 16, immunoreactions of fibronectin in the apical ectodermal ridge were localized on the plasma membranes of epidermal cells and cytoplasmic projections of the underlying mesenchymal cells, which are in contact with the basal lamina. Those of integrin alpha 3 and alpha 5 subunits also appeared on such areas. Definite immunoreactions of fibronectin and both integrin subunits were seen in cell to cell contact areas of mesenchymal cells which are associated with the marginal vein, or with each other forming solid cell cords, and appeared on the basal plasma membrane of endothelial cells of the growing capillaries arising from the marginal vein. These findings suggest that fibronectin may work as a ligand for alpha 3 beta 1 and/or alpha 5 beta 1 integrins expressed by the mesenchymal and vasoformative cells in developing limb bud.
在胚胎发育第14至16天的大鼠后肢芽中，纤连蛋白在顶端外胚层嵴的免疫反应定位于表皮细胞的质膜以及与基膜接触的下方间充质细胞的细胞质突起上。整合素α3和α5亚基的免疫反应也出现在这些区域。在与边缘静脉相关的间充质细胞的细胞间接触区域，或彼此形成实心细胞索的间充质细胞的细胞间接触区域，以及出现在由边缘静脉产生的正在生长的毛细血管内皮细胞的基底质膜上，可见纤连蛋白和两种整合素亚基的明确免疫反应。这些发现表明，纤连蛋白可能作为由发育中的肢芽中的间充质细胞和血管形成细胞表达的α3β1和/或α5β1整合素的配体发挥作用。
