Yamato M, Nakada R, Nakamura Y
Department of Food Microbiology, School of Medicine, University of Tokushima, Japan.
Microbiol Res. 1998 Apr;153(1):29-35. doi: 10.1016/S0944-5013(98)80018-9.
We have studied the effect of medium components on spirosin production in Lactobacillus reuteri DSM 20016 to get some insight into the function of spirosome in Lactobacillus. Among those investigated, only potassium phosphate was found to influence the production of spirosin. Though it was not effective at concentrations lower than 0.6%, it promoted the spirosin production and its accumulation into culture medium in particular, at higher concentrations. The accumulation has been demonstrated to originate inthe release of spirosin due to the bacterial autolysis induced by the higher concentrations of potassium phosphate, The autolytic effect of potassium phosphate was assumed to be a concerted action by phosphate and potassium ions at the concentrations higher than 0.1 and 0.4 M, respectively. A possible role of spirosin as a sensor protein of bacterial two-component regulatory system was discussed.
我们研究了培养基成分对罗伊氏乳杆菌DSM 20016中螺菌素产生的影响，以深入了解螺体在乳杆菌中的功能。在所研究的成分中，仅发现磷酸钾会影响螺菌素的产生。尽管在浓度低于0.6%时无效，但在较高浓度下，它尤其能促进螺菌素的产生及其在培养基中的积累。已证明这种积累源于较高浓度的磷酸钾诱导细菌自溶而导致的螺菌素释放。磷酸钾的自溶作用被认为是分别在高于0.1 M和0.4 M的浓度下，磷酸根离子和钾离子的协同作用。本文还讨论了螺菌素作为细菌双组分调节系统传感蛋白的可能作用。
