Anbazhagan R, Osin P P, Bartkova J, Nathan B, Lane E B, Gusterson B A
Section of Cell Biology and Experimental Pathology, Institute of Cancer Research, Haddow Laboratories, Sutton, Surrey, U.K.
J Pathol. 1998 Feb;184(2):197-206. doi: 10.1002/(SICI)1096-9896(199802)184:2<197::AID-PATH992>3.0.CO;2-J.
In order to explain the molecular events that contribute to benign and malignant breast disease, it is essential to understand the cellular context in which these are occurring. This study describes a detailed analysis of the epithelial phenotypes in the human fetal and infant breast and provides a starting point for such consideration. Using methacarn-fixed, paraffin sections from ten fetal and 45 infant breast, immunostained with a panel of antibodies to cytoskeletal proteins and kappa-casein, it has been possible to define in detail the chronological evolution of the major cell types in the human breast from 16 weeks of intrauterine life to 2 years of age, in both sexes. Cells at the tips of the lobular buds and terminal end buds have a characteristic cytoskeletal protein profile, suggesting that they may have the capacity to generate both basal cells and luminal cells. Based on the expression of cytoskeletal proteins in the developing fetal and infant breast, a model system has been proposed for mammary epithelial differentiation.
为了解释导致乳腺良性和恶性疾病的分子事件，必须了解这些事件发生的细胞背景。本研究描述了对人类胎儿和婴儿乳腺上皮表型的详细分析，并为这种考量提供了一个起点。使用来自10例胎儿和45例婴儿乳腺的甲醇 Carnoy 固定石蜡切片，用一组针对细胞骨架蛋白和κ-酪蛋白的抗体进行免疫染色，得以详细界定人类乳腺中主要细胞类型从子宫内生活16周直至2岁时按时间顺序的演变，涵盖男女两性。小叶芽尖和终末芽处的细胞具有特征性的细胞骨架蛋白谱，表明它们可能具有产生基底细胞和管腔细胞的能力。基于发育中的胎儿和婴儿乳腺中细胞骨架蛋白的表达，提出了一个乳腺上皮分化的模型系统。
