Nagira M, Imai T, Yoshida R, Takagi S, Iwasaki M, Baba M, Tabira Y, Akagi J, Nomiyama H, Yoshie O
Shionogi Institute for Medical Science, Osaka, Japan.
Eur J Immunol. 1998 May;28(5):1516-23. doi: 10.1002/(SICI)1521-4141(199805)28:05<1516::AID-IMMU1516>3.0.CO;2-J.
Secondary lymphoid tissue chemokine (SLC) is a CC chemokine expressed mainly in lymph nodes, appendix and spleen, and specifically chemotactic for lymphocytes (Nagira et al., J. Biol. Chem. 1997. 272: 19518-19524). Here, we carried out transendothelial migration assays to determine the classes and subsets of lymphocytes migrating toward SLC. SLC attracted freshly isolated B cells with high efficiency and T cells modestly. Thus, SLC is the first CC chemokine with a strong chemotactic activity on fresh B cells. Among T cell types and subsets, SLC broadly attracted CD4+ and CD8+ cells, CD45RO- (naive) and CD45RO+ (memory) cells, and CD26high (activated) and CD26low- (resting) cells. SLC also attracted both L-selectin+ and L-selectin- subpopulations of various T cell subsets and B cells. Furthermore, mitogenic stimulation strongly enhanced migratory responses of T cells and B cells toward SLC. By in situ hybridization, SLC mRNA was detected in the cortical parafollicular regions (the T cell areas) of a lymph node and an appendix. Collectively, SLC may be a basic chemokine supporting homeostatic migration of a broad spectrum of lymphocytes into the secondary lymphoid tissues. SLC may also be involved in immune responses by inducing highly efficient migration of T and B cells following antigenic stimulation.
二级淋巴组织趋化因子（SLC）是一种CC趋化因子，主要在淋巴结、阑尾和脾脏中表达，对淋巴细胞具有特异性趋化作用（Nagira等人，《生物化学杂志》，1997年。272:19518 - 19524）。在此，我们进行了跨内皮迁移试验，以确定向SLC迁移的淋巴细胞的类别和亚群。SLC高效吸引新鲜分离的B细胞，对T细胞也有一定程度的吸引。因此，SLC是首个对新鲜B细胞具有强趋化活性的CC趋化因子。在T细胞类型和亚群中，SLC广泛吸引CD4 +和CD8 +细胞、CD45RO -（幼稚）和CD45RO +（记忆）细胞，以及CD26高（活化）和CD26低 -（静止）细胞。SLC还吸引各种T细胞亚群和B细胞的L - 选择素 +和L - 选择素 -亚群。此外，丝裂原刺激强烈增强T细胞和B细胞对SLC的迁移反应。通过原位杂交，在淋巴结和阑尾的皮质滤泡旁区域（T细胞区）检测到SLC mRNA。总体而言，SLC可能是一种基础趋化因子，支持广泛的淋巴细胞向二级淋巴组织的稳态迁移。SLC也可能通过在抗原刺激后诱导T细胞和B细胞的高效迁移而参与免疫反应。
