Duraisingh M T, Curtis J, Warhurst D C
Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, United Kingdom.
Exp Parasitol. 1998 May;89(1):1-8. doi: 10.1006/expr.1998.4274.
With the spread of resistance to chloroquine, the combination of sulphadoxine and pyrimethamine is growing in importance for the treatment of infection with the malaria parasite Plasmodium falciparum. Mutations in the dhfr gene of P. falciparum have been associated with resistance to pyrimethamine. Recently, several polymorphisms have been identified in the P. falciparum dhps gene which may correlate with sulphadoxine-resistance. Simple and rapid tests have been developed to detect these polymorphisms, using PCR followed by restriction digestion. These tests can accurately identify all the polymorphisms described to date at codons 16, 51, 59, 108, and 164 in the dhfr gene and those at codons 436, 437, 540, 581, and 613 in the dhps gene. A nested system has been developed which allows the accurate detection of these polymorphisms in samples of fingerprick blood collected on glass fiber membranes and filter papers, some with very low parasitaemias.
随着对氯喹耐药性的传播，磺胺多辛与乙胺嘧啶的联合用药在治疗恶性疟原虫感染方面的重要性日益增加。恶性疟原虫二氢叶酸还原酶（dhfr）基因的突变与对乙胺嘧啶的耐药性有关。最近，在恶性疟原虫二氢蝶酸合酶（dhps）基因中发现了几种多态性，这些多态性可能与对磺胺多辛的耐药性相关。已开发出简单快速的检测方法来检测这些多态性，方法是先进行聚合酶链反应（PCR），然后进行限制性酶切。这些检测方法能够准确识别迄今所描述的dhfr基因中第16、51、59、108和164密码子以及dhps基因中第436、437、540、581和613密码子处的所有多态性。已开发出一种巢式系统，可准确检测在玻璃纤维膜和滤纸上采集的微量血样中的这些多态性，其中一些血样的疟原虫血症水平非常低。
