Vanbever R, Fouchard D, Jadoul A, De Morre N, Préat V, Marty J P
Unité de Pharmacie Galénique, Université catholique de Louvain, Bruxelles, Belgique.
Skin Pharmacol Appl Skin Physiol. 1998 Jan-Feb;11(1):23-34. doi: 10.1159/000029805.
Short high-voltage pulses have recently been shown to dramatically increase and expedite transdermal drug transport via a mechanism hypothesized to involve electroporation. This study addresses tolerance issues of the method in vivo in hairless rat. Chromametry, transepidermal water loss (TEWL), laser Doppler flowmetry (LDF) and corneometry were jointly used for noninvasive sensing of skin biophysical parameters. Slight increases in skin redness, TEWL and LDF values followed the application of electric pulses. The changes in skin capacitance were nonsignificant. The magnitude of the alterations depended on the electrical features of the pulses. When compared to iontophoresis, high-voltage pulses did not induce stronger alterations of skin functions. This report provides the first in vivo demonstration of the safety of the high-voltage pulses proposed for transdermal delivery.
近期研究表明，短高压脉冲可通过一种推测涉及电穿孔的机制，显著增加并加速经皮药物转运。本研究探讨了该方法在无毛大鼠体内的耐受性问题。联合使用色度测定法、经表皮水分流失（TEWL）、激光多普勒血流仪（LDF）和角质层含水量测定仪，对皮肤生物物理参数进行无创检测。施加电脉冲后，皮肤发红、TEWL和LDF值略有增加。皮肤电容的变化不显著。改变的幅度取决于脉冲的电学特性。与离子电渗疗法相比，高压脉冲并未引起更强的皮肤功能改变。本报告首次在体内证明了用于经皮给药的高压脉冲的安全性。
