Chubinskaya S, Cs-Szabo G, Kuettner K E
Department of Biochemistry, Rush Medical College at Rush-Presbyterian-St Luke's Medical Center, Chicago, Illinois 60612, USA.
J Histochem Cytochem. 1998 Jun;46(6):723-9. doi: 10.1177/002215549804600604.
The depletion of the pericellular and territorial matrices in articular cartilage is considered to be one of the earliest events in pathobiology of osteoarthritis (OA). A newly discovered family of proteins with a disintegrin-like and metalloproteinase-like domain (ADAM) may be involved in matrix degradation as well as in cell-cell and cell-matrix interactions. The purpose of this study was to determine by in situ hybridization whether human articular chondrocytes from newborn, normal adult, and OA cartilages express messenger RNA for ADAM-10, one of the members of this family, and by semiquantitative RT-PCR to compare the levels of this expression. The results confirmed the expression of ADAM-10 by human articular chondrocytes and revealed the highest levels of expression in the continuously remodeling cartilage of newborns and the most fibrillated areas of OA cartilage, especially the regions of cell clusters. Importantly, ADAM-10 mRNA expression was evident in tissues with the greatest loss of Safranin O staining from the territorial and interterritorial matrix of the chondrocytes. Messenger RNA was upregulated in OA tissue compared to the age-matched normal cartilage, as detected by RT-PCR. Upregulated levels of ADAM-10 mRNA expression appear to be related to the degree of cartilage damage and/or degradation, which suggests a potential role for at least one member of this new family in the cartilage matrix destruction accompanying OA.
关节软骨中细胞周围和局部基质的消耗被认为是骨关节炎（OA）病理生物学中最早出现的事件之一。新发现的一类具有解整合素样和金属蛋白酶样结构域的蛋白质（ADAM）可能参与基质降解以及细胞 - 细胞和细胞 - 基质相互作用。本研究的目的是通过原位杂交确定来自新生儿、正常成年人和OA软骨的人关节软骨细胞是否表达该家族成员之一ADAM - 10的信使RNA，并通过半定量RT - PCR比较这种表达水平。结果证实了人关节软骨细胞表达ADAM - 10，并显示在新生儿持续重塑的软骨以及OA软骨最纤维化的区域，尤其是细胞簇区域，ADAM - 10的表达水平最高。重要的是，在软骨细胞的局部和区域间基质中番红O染色损失最大的组织中，ADAM - 10 mRNA表达明显。通过RT - PCR检测，与年龄匹配的正常软骨相比，OA组织中的信使RNA上调。ADAM - 10 mRNA表达上调的水平似乎与软骨损伤和/或降解程度有关，这表明该新家族的至少一个成员在伴随OA的软骨基质破坏中可能发挥作用。
