Kassner P D, Conroy W G, Berg D K
Department of Biology, University of California at San Diego, La Jolla 92093, USA.
Mol Cell Neurosci. 1998 Apr;10(5-6):258-70. doi: 10.1006/mcne.1998.0664.
Targeting receptors to appropriate locations on the cell surface is a critical task for neurons. We have examined the possibility that rapsyn controls the distribution of nicotinic receptors on neurons as it does nicotinic receptors on muscle fibers. Cotransfection of QT6 cells with rapsyn and neuronal nicotinic receptor cDNA constructs produced receptor aggregates or clusters that codistributed in part with rapsyn protein. Though all nicotinic receptor subtypes tested were affected by rapsyn, receptors containing the alpha 7 gene product were among the most responsive. In addition, rapsyn caused a portion of the nicotinic receptors containing alpha 7 subunits to become resistant to solubilization with nonionic detergent and to display a marked increase in metabolic stability. The results are consistent with rapsyn linking the receptors to cytoskeletal elements and suggest that it may play an organizing role determining the fate and location of nicotinic receptors on neurons.
将受体定位到细胞表面的适当位置是神经元的一项关键任务。我们研究了rapsyn是否像控制肌肉纤维上的烟碱型受体那样，控制神经元上烟碱型受体的分布。将rapsyn与神经元烟碱型受体cDNA构建体共转染QT6细胞，产生了受体聚集体或簇，它们部分与rapsyn蛋白共分布。尽管所有测试的烟碱型受体亚型都受到rapsyn的影响，但含有α7基因产物的受体反应最为强烈。此外，rapsyn使一部分含有α7亚基的烟碱型受体对非离子去污剂的溶解产生抗性，并使其代谢稳定性显著增加。这些结果与rapsyn将受体与细胞骨架成分联系起来一致，并表明它可能在决定神经元上烟碱型受体的命运和位置方面发挥组织作用。
