Solntseva E I
Science Research Institute of the Brain, Russian Academy, Medical Sciences, Moscow.
Neurosci Behav Physiol. 1998 Mar-Apr;28(2):116-20. doi: 10.1007/BF02461956.
A two-microelectrode potential clamping method was used on isolated common snail neurons to measure high-threshold Ca2+ and delayed rectified K+ currents. Addition of the nootropic agent vinpocetine (VPC) to the bathing solution rapidly and reversibly inhibited both types of current. The effects of VPC were dose-dependent and were independent of the test stimulus voltage. Maximum blockade of the Ca2+ current averaged 27% at a VPC concentration of 600 microM. Maximum blockade of the K+ current averaged 76% at a VPC concentration of 30 microM. It is concluded that K+ channels are more likely targets of VPC than Ca2+ channels.
采用双微电极电压钳制方法，在分离的普通蜗牛神经元上测量高阈值Ca2+电流和延迟整流K+电流。向浴液中添加促智药长春西汀（VPC）可快速且可逆地抑制这两种电流。VPC的作用呈剂量依赖性，且与测试刺激电压无关。在VPC浓度为600 microM时，Ca2+电流的最大阻断平均为27%。在VPC浓度为30 microM时，K+电流的最大阻断平均为76%。得出的结论是，与Ca2+通道相比，K+通道更可能是VPC的作用靶点。
