黑曲霉和禾谷镰刀菌对呋喃丹的代谢作用

Metabolism of carbofuran by Aspergillus niger and Fusarium graminearum.

作者信息

Salama A K

机构信息

Plant Protection Department, College of Agric. & Vet. Med., King Saud University, Qasseem Branch, Bureidah, Saudi Arabia.

出版信息

J Environ Sci Health B. 1998 May;33(3):253-66. doi: 10.1080/03601239809373142.

DOI:10.1080/03601239809373142

PMID:9604338

Abstract

Metabolism of carbofuran in pure liquid cultures of A. niger and F. graminearum was investigated. Carbofuran and its metabolites were analyzed by HPLC and TLC. The average recoveries of carbofuran, 3-hydroxycarbofuran, 3-ketocarbofuran, and 3-ketocarbofuran phenol from fungi media were found to be 88.65, 86.19, 75.48, and 80.48%, while their detection limits were 0.035, 0.031, 0.015 and 0.140 ppm, respectively. The data showed that A. niger was capable of degrading carbofuran more faster than F. graminearum. Carbofuran disappeared biexponentially from the liquid culture media of both fungi. The terminal half-life values of carbofuran were 10.4 and 12 days in the media of A. niger and F. graminearum, respectively. The amounts of carbofuran reached 15.56 and 19.71% of the applied dose after 21 days in case of A. niger and F. graminearum, respectively. Carbofuran was biotransformed to 3-hydroxycarbofuran, 3-ketocarbofuran, and 3-ketocarbofuran phenol. The percentages of the major metabolite, 3-hydroxycarbofuran were 47.72 and 7.77% in case of A. niger and F. graminearum after 21 days, respectively.

摘要

研究了克百威在黑曲霉和禾谷镰刀菌纯液体培养物中的代谢情况。通过高效液相色谱法（HPLC）和薄层色谱法（TLC）对克百威及其代谢产物进行了分析。从真菌培养基中回收克百威、3 - 羟基克百威、3 - 酮基克百威和3 - 酮基克百威苯酚的平均回收率分别为88.65%、86.19%、75.48%和80.48%，而它们的检测限分别为0.035 ppm、0.031 ppm、0.015 ppm和0.140 ppm。数据表明，黑曲霉降解克百威的速度比禾谷镰刀菌更快。克百威从两种真菌的液体培养基中呈双指数消失。在黑曲霉和禾谷镰刀菌的培养基中，克百威的终末半衰期值分别为10.4天和12天。在21天后，黑曲霉和禾谷镰刀菌培养基中克百威的含量分别达到施用量的15.56%和19.71%。克百威被生物转化为3 - 羟基克百威、3 - 酮基克百威和3 - 酮基克百威苯酚。在21天后，黑曲霉和禾谷镰刀菌中主要代谢产物3 - 羟基克百威的百分比分别为47.72%和7.77%。