Coomber D W, Hawkins N J, Dalley D, Ward R L
Department of Medical Oncology, St Vincent's Hospital, Sydney, NSW, Australia.
Neoplasma. 1998;45(1):12-6.
The sera of 54 individuals with colorectal or breast cancer, and 50 healthy volunteers were assayed for the presence of anti-bovine submaxillary mucin antibodies using an enzyme linked immunoassay. The serum levels of these antibodies were found to be significantly lower in people with breast (p < 0.001) or colorectal cancer (p < 0.001) with respect to healthy individuals. Within the colorectal cancer group the presence of antibodies was significantly lower in those individuals with poorly differentiated tumors compared to other histological grades (p < 0.05), but did not correlate with the presence of local or distant metastases or anatomical location of the tumor (p > 0.05). No correlation was found with respect to the age of the patient and the level of anti-sialyl-Tn antibodies (p > 0.05). Competition analysis with the anti-sialyl-Tn monoclonal antibody 3C2 indicated that the activity against bovine submaxillary mucin was primarily due to specificity for the sialyl-Tn epitope of the glycoprotein. In contrast to findings with other tumor associated antigens, we could find no evidence of an increase in the level of antibodies against this epitope.
采用酶联免疫分析法检测了54例结直肠癌或乳腺癌患者以及50名健康志愿者血清中抗牛颌下粘蛋白抗体的存在情况。结果发现，乳腺癌患者（p < 0.001）或结直肠癌患者（p < 0.001）血清中这些抗体的水平显著低于健康个体。在结直肠癌组中，与其他组织学分级相比，低分化肿瘤患者体内抗体的存在显著降低（p < 0.05），但与局部或远处转移的存在或肿瘤的解剖位置无关（p > 0.05）。未发现患者年龄与抗唾液酸-Tn抗体水平之间存在相关性（p > 0.05）。与抗唾液酸-Tn单克隆抗体3C2的竞争分析表明，针对牛颌下粘蛋白的活性主要归因于对糖蛋白唾液酸-Tn表位的特异性。与其他肿瘤相关抗原的研究结果相反，我们没有发现针对该表位的抗体水平升高的证据。
