非小细胞肺癌中血管内皮生长因子的表达：对鳞状细胞癌的预后意义

Vascular endothelial growth factor expression in non-small-cell lung cancer: prognostic significance in squamous cell carcinoma.

作者信息

Imoto H, Osaki T, Taga S, Ohgami A, Ichiyoshi Y, Yasumoto K

机构信息

Department of Surgery II, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.

出版信息

J Thorac Cardiovasc Surg. 1998 May;115(5):1007-14. doi: 10.1016/S0022-5223(98)70398-8.

DOI:10.1016/S0022-5223(98)70398-8

PMID:9605068

Abstract

BACKGROUND

Recently, some studies have focused on the tumor angiogenesis and its prognostic value. We studied the expression of vascular endothelial growth factor, microvessel counts, and serum concentrations of vascular endothelial growth factor to investigate their association with clinicopathologic factors and prognosis in non-small-cell lung cancer.

METHODS

The expression of vascular endothelial growth factor was determined by an immunohistochemical analysis from 91 paraffin specimens of completely resected non-small-cell lung cancers using anti-growth factor polyclonal antibody. Microvessel staining was performed by immunohistochemical analysis with anti-factor VIII-related antigen polyclonal antibody. Measurement of the serum concentrations of vascular endothelial growth factor used the sandwich enzyme-linked immunosorbent assay technique.

RESULTS

Expression of vascular endothelial growth factor was detected in 48 of the 91 tumors. The positive ratio was significantly higher in patients with adenocarcinoma than in those with squamous cell carcinoma. The microvessel counts were significantly higher in the patients with nodal metastasis than in those without nodal metastasis. The serum concentrations of vascular endothelial growth factor were also significantly higher in the patients with T3-4 disease than in those with T1-2 disease. The microvessel counts were closely associated with expression of vascular endothelial growth factor. The prognosis of patients with a positive growth factor ratio was significantly worse than that of the patients with a negative ratio (p = 0.002), especially in squamous cell carcinoma. According to a multivariate analysis, only nodal status and expression of vascular endothelial growth factor were found to be independent prognostic factors.

CONCLUSIONS

The expression of vascular endothelial growth factor was one of the most important prognostic factors in completely resected non-small-cell lung cancer, especially in squamous cell carcinoma.

摘要

背景

最近，一些研究聚焦于肿瘤血管生成及其预后价值。我们研究了血管内皮生长因子的表达、微血管计数以及血管内皮生长因子的血清浓度，以探讨它们与非小细胞肺癌临床病理因素及预后的关系。

方法

采用抗生长因子多克隆抗体，通过免疫组织化学分析对91例完全切除的非小细胞肺癌石蜡标本进行血管内皮生长因子表达的测定。使用抗因子VIII相关抗原多克隆抗体通过免疫组织化学分析进行微血管染色。采用夹心酶联免疫吸附测定技术测量血管内皮生长因子的血清浓度。

结果

91例肿瘤中有48例检测到血管内皮生长因子的表达。腺癌患者的阳性率显著高于鳞状细胞癌患者。有淋巴结转移的患者微血管计数显著高于无淋巴结转移的患者。T3 - 4期疾病患者的血管内皮生长因子血清浓度也显著高于T1 - 2期疾病患者。微血管计数与血管内皮生长因子的表达密切相关。生长因子阳性率患者的预后明显差于阴性率患者（p = 0.002），尤其是在鳞状细胞癌中。多因素分析显示，只有淋巴结状态和血管内皮生长因子的表达是独立的预后因素。