Kusumoto K I, Hino A, Yonekura Y, Nogata Y, Ohta H, Takano H
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Tsukuba, Ibaraki, Japan.
Yeast. 1998 Apr 30;14(6):583-6. doi: 10.1002/(SICI)1097-0061(19980430)14:6<583::AID-YEA245>3.0.CO;2-4.
Homologs of Saccharomyces cerevisiae STE3, a-factor receptor gene were detected from S. exiguus NFRI 3539 by low stringency Southern hybridization. This strain might have at least two types of homolog. One of these homologs, designated as e-STE3 was cloned. Its nucleotide sequence revealed 60% identity to STE3. The putative protein coding region consisted of 453 amino acid residues. The amino acid sequence identity between STE3 and e-STE3 was 62% and that of the N-terminal 303 amino acid residues considered to be the pheromone binding domain was 79%.
通过低严谨度Southern杂交，从小球拟酵母NFRI 3539中检测到酿酒酵母STE3（α-因子受体基因）的同源物。该菌株可能至少有两种同源物。其中一种同源物被命名为e-STE3并被克隆。其核苷酸序列与STE3有60%的同一性。推定的蛋白质编码区由453个氨基酸残基组成。STE3和e-STE3之间的氨基酸序列同一性为62%，而被认为是信息素结合结构域的N端303个氨基酸残基的同一性为79%。
