Dikova Ts
Vet Med Nauki. 1976;13(5):44-8.
Studied were the morphologic and biochemic properties of 85 Pasteurella haemolytica strains belonging to the known 12 serotypes after Biberstein, Gills (2). The biochemical properties of the strains were studied after the pattern of Bosworth and Lovell (4) with regard to arabinose, trehalose, xylose, manose, salysin, and lactose. It was found that the biochemical properties correlated with the biotype classification of the strains. Thus, the biochemical reaction with trehalose and arabinose may be used as a characteristic feature in determining the biotype. The reaction proved more dependable with the strains of the T biotype as compared to those of the A biotype, as not all strains of the A biotype fermented arabinose in the course of the first seven days, while with the strains of the T biotype trehalose was acted upon during such a period. It was also established that xylose is attacked by the strains of the A biotype only, and manose and salycin--by the strains of the T biotype, which did not ferment lactose. All strains of Pasteurella haemolytica turned out to be negative so far as the production and indol was concerned; they were shown to reduce the nitrates into nitrites, were MP and VP negative, and gave growth in McConkey's agar medium.
对属于比伯斯坦、吉尔斯(2)所确定的12种血清型的85株溶血巴斯德氏菌的形态学和生物化学特性进行了研究。按照博斯沃思和洛弗尔(4)的方法,研究了这些菌株关于阿拉伯糖、海藻糖、木糖、甘露糖、沙雷菌素和乳糖的生物化学特性。发现这些生物化学特性与菌株的生物型分类相关。因此,与海藻糖和阿拉伯糖的生物化学反应可作为确定生物型的一个特征。与A生物型菌株相比,该反应在T生物型菌株中更可靠,因为并非所有A生物型菌株在前七天内都能发酵阿拉伯糖,而T生物型菌株在此期间能分解海藻糖。还确定只有A生物型菌株能分解木糖,而T生物型菌株能分解甘露糖和沙雷菌素,且T生物型菌株不发酵乳糖。就吲哚的产生而言,所有溶血巴斯德氏菌菌株均呈阴性;它们能将硝酸盐还原为亚硝酸盐,甲基红试验和V-P试验均为阴性,且能在麦康凯琼脂培养基中生长。