Gargioni R, Barracco M A
Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, Florianópolis, Brazil.
J Morphol. 1998 Jun;236(3):209-21. doi: 10.1002/(SICI)1097-4687(199806)236:3<209::AID-JMOR4>3.0.CO;2-Y.
The hemocytes of two palaemonids and one penaeid were characterized using light and transmission electron microscopy (TEM). The blood cells in all three species were classified as hyaline hemocytes (HH), small granule hemocytes (SGH), and large granule hemocytes (LGH). The HH are unstable hemocytes with a characteristic high nucleo-cytoplasmic ratio. Their cytoplasm appears particularly dense and has from few to numerous granules that often exhibit a typical striated substructure. In both palaemonids, the great majority of the HH contain numerous granules, whereas in Penaeus paulensis, a small number of these cells have few or no granules. The cytoplasm of some HH of the penaeid exhibits typical electron-dense deposits. The granulocytes, LGH and SGH, contain abundant electron-dense granules that are usually smaller in the SGH. In both hemocyte types, the cytosol, but not the granules, is rich in carbohydrates (PAS positive) and numerous vesicles contain acid phosphatase (Gomori reactive). In all studied shrimps, the SGH and LGH were actively phagocytic when examined on blood cell monolayers incubated with the yeast Saccharomyces cerevisiae. A few mitotic figures (less than 1%) were observed in the granulocytes of P. paulensis, but not in the palaemonids. SGH is the main circulating blood cell type in both palaemonids, whereas HH is predominant in the penaeid. Based on morphological and functional features, it appears that the hyaline and the granular hemocytes of the three shrimp species represent different cell lineages.
利用光学显微镜和透射电子显微镜（TEM）对两种长臂虾科和一种对虾科虾类的血细胞进行了特征描述。这三个物种的血细胞都被分类为透明血细胞（HH）、小颗粒血细胞（SGH）和大颗粒血细胞（LGH）。HH是不稳定的血细胞，具有高核质比的特征。它们的细胞质显得特别致密，有少量到大量的颗粒，这些颗粒通常呈现出典型的条纹状亚结构。在两种长臂虾科中，绝大多数HH含有大量颗粒，而在保罗对虾中，少数这类细胞几乎没有或没有颗粒。对虾科一些HH的细胞质呈现出典型的电子致密沉积物。粒细胞（LGH和SGH）含有丰富的电子致密颗粒，SGH中的颗粒通常较小。在这两种血细胞类型中，细胞溶胶富含碳水化合物（过碘酸希夫反应阳性），而颗粒不含，并且许多囊泡含有酸性磷酸酶（Gomori反应阳性）。在所有研究的虾类中，当在与酿酒酵母一起孵育的血细胞单层上进行检查时，SGH和LGH具有活跃的吞噬作用。在保罗对虾粒细胞中观察到少数有丝分裂图像（不到1%），但在长臂虾科中未观察到。SGH是两种长臂虾科中主要的循环血细胞类型，而HH在对虾科中占主导地位。基于形态和功能特征，这三种虾类的透明血细胞和颗粒血细胞似乎代表不同的细胞谱系。
