Bohnemeier H, Pinto Y M, Horkay F, Tóth M, Juhász-Nagy A, Orzechowski H D, Böhm M, Paul M
Department of Clinical Pharmacology, Benjamin Franklin University Hospital, Free University of Berlin, Germany.
Clin Exp Hypertens. 1998 May;20(4):417-37. doi: 10.3109/10641969809053222.
Endothelin-1 converting-enzyme (ECE-1) cleaves the precursor, big-endothelin-1, to the active peptide endothelin-1. The aim of this study was to investigate whether ECE-1 mRNA expression is modified in human cardiovascular disease. Tissue samples from the left human atrium were analyzed for ECE-1 expression and related to different clinical parameters. A quantitative PCR assay (qPCR) with competitive and non-competitive standards was established. The ECE-1 measurements were normalized by a GAPDH qPCR. Patients who suffered from a myocardial infarction had elevated ECE-1 levels when compared to controls (5.81+/-0.76 vs. 3.20+/-0.51 fg ECE-1, ng GAPDH, p<0.05). Drug treatment with the beta-blocker metoprolol was associated with a decreased ECE-1 expression level (3.90+/-0.58 vs. 5.81+/-0.76 fg ECE-1, ng GAPDH, p<0.1). We conclude that the expression of ECE-1 is altered in the atrial tissue depending on the physiological status of the heart. This suggests a differential role of ECE-1 in cardiovascular diseases.
内皮素 -1 转换酶（ECE -1）可将前体大内皮素 -1 切割为活性肽内皮素 -1。本研究旨在探讨在人类心血管疾病中 ECE -1 mRNA 的表达是否发生改变。对取自人类左心房的组织样本进行 ECE -1 表达分析，并与不同临床参数相关联。建立了带有竞争性和非竞争性标准品的定量 PCR 检测方法（qPCR）。通过 GAPDH qPCR 对 ECE -1 的测量值进行标准化。与对照组相比，心肌梗死患者的 ECE -1 水平升高（5.81±0.76 对 3.20±0.51 fg ECE -1/ng GAPDH，p<0.05）。使用β受体阻滞剂美托洛尔进行药物治疗与 ECE -1 表达水平降低相关（3.90±0.58 对 5.81±0.76 fg ECE -1/ng GAPDH，p<0.1）。我们得出结论，ECE -1 的表达在心房组织中根据心脏的生理状态而改变。这表明 ECE -1 在心血管疾病中具有不同的作用。
