Fox N E, van Kuijk F J
Department of Chemistry and Biochemistry, Montana State University, Bozeman, USA.
Free Radic Biol Med. 1998 Apr;24(6):900-5. doi: 10.1016/s0891-5849(97)00369-9.
Xanthine oxidase has been established as an important source of oxygen free radicals in ischemia-reperfusion injury. It has been localized in many different tissues such as heart and intestine, but it has not yet been localized in the eye. Xanthine oxidase was detected using immunohistochemistry on paraformaldehyde/glutaraldehyde fixed cryosections. Antibodies used included rabbit antibovine xanthine oxidase antibody and rabbit antihuman xanthine oxidase antibody. Xanthine oxidase was detected in the capillary endothelium cells of blood vessels in the retina of bovine and post mortem human eyes. Whole mount preparation of human retinas showed xanthine oxidase present throughout the small capillary network. Furthermore, whole mounts showed that xanthine oxidase was present in cones. This was confirmed by using mouse anticalbindin antibody for co-labelling. It is possible that xanthine oxidase can be a source of oxidative damage in the retina following ischemia-reperfusion injury.
黄嘌呤氧化酶已被确认为缺血再灌注损伤中氧自由基的重要来源。它已在许多不同组织（如心脏和肠道）中定位，但尚未在眼部定位。在经多聚甲醛/戊二醛固定的冰冻切片上使用免疫组织化学检测黄嘌呤氧化酶。使用的抗体包括兔抗牛黄嘌呤氧化酶抗体和兔抗人黄嘌呤氧化酶抗体。在牛眼和人死后眼睛的视网膜血管内皮细胞中检测到黄嘌呤氧化酶。人视网膜整装标本显示黄嘌呤氧化酶存在于整个小毛细血管网络中。此外，整装标本显示黄嘌呤氧化酶存在于视锥细胞中。这通过使用小鼠抗钙结合蛋白抗体进行共标记得到了证实。缺血再灌注损伤后，黄嘌呤氧化酶有可能成为视网膜氧化损伤的一个来源。
