通过皮肤、肌肉或内脏神经逆行标记的大鼠初级传入神经元中寡糖、神经肽、碳酸酐酶和神经丝表达的差异。

Differences in expression of oligosaccharides, neuropeptides, carbonic anhydrase and neurofilament in rat primary afferent neurons retrogradely labelled via skin, muscle or visceral nerves.

作者信息

Perry M J, Lawson S N

机构信息

Department of Physiology, The School of Medical Sciences, Bristol, UK.

出版信息

Neuroscience. 1998 Jul;85(1):293-310. doi: 10.1016/s0306-4522(97)00629-5.

DOI:10.1016/s0306-4522(97)00629-5

PMID:9607720

Abstract

Dorsal root ganglion neurons innervating skin via the saphenous nerve, muscle via the gastrocnemius nerve and viscera via the splanchnic nerve, were identified by retrograde tracing with Fast Blue applied to the cut nerve. Only neuronal profiles with nuclei were counted. At the survival times used no changes in immunohistochemical labelling patterns were detectable in the axotomized neurons. Percentages of Fast Blue-labelled neuronal profiles that were immunolabelled were calculated. The values for markers of carbohydrate groups were for skin, muscle and viscera, respectively: the lectin peanut agglutinin 55%, 24%, and 50%; the lectin soybean agglutinin 72%, 56%, 61%; the antibody 2C5 (against lactoseries groups) 43%, 20%, 6%; the antibodies SSEA-4 (against globoseries groups) 6%, 12%, 0% and SSEA-3 (against globoseries groups) 6%, 5%, 0%. The values for neurofilament rich profiles were for skin, muscle and viscera, respectively: 34%, 43%, 19%, and for carbonic anhydrase were 10%, 33%, 2%. Values for neuropeptides were, for calcitonin gene-related peptide 51%, 70%, 99%, for substance P 21%, 51%, 82%, and for somatostatin 10%, 2% and 0%. The population of skin afferents therefore contained the highest proportion of profiles expressing galactose containing carbohydrate groups labelled by 2C5 and the lectins and the highest proportion of cells with somatostatin. In contrast they had the lowest proportions of cells with calcitonin gene-related peptide and substance P, compared with the other tissues. Muscle afferents had the highest proportions compared with the other tissues of the neurofilament-rich, carbonic anhydrase-positive and SSEA-4-labelled profiles, but the lowest proportions of profiles with lectin binding. The splanchnic visceral afferents had the highest proportions, compared with the other tissues, of neuronal profiles labelled for calcitonin gene-related peptide and substance P, but the lowest proportions of neurofilament rich profiles and of profiles with carbonic anhydrase or 2C5 labelling and they totally lacked any labelling for globoseries carbohydrates and somatostatin. Both the muscle and skin afferent populations had clear small cell and large cell peaks in their size distributions, with the small cell peak being larger for skin than muscle afferents and the large cell peak being more marked for muscle afferents. The visceral afferent profiles had a unimodal size distribution with the peak size being between the small and large cell peaks of the somatic afferent units. This study therefore shows that the patterns of immunohistochemical labelling and cell size of primary afferent neurons differ according to their peripheral target tissue.

摘要

通过将快蓝逆行追踪应用于切断的神经，鉴定出经隐神经支配皮肤、经腓肠肌神经支配肌肉以及经内脏神经支配内脏的背根神经节神经元。仅对有细胞核的神经元轮廓进行计数。在所使用的存活时间内，未检测到轴突切断的神经元免疫组化标记模式有变化。计算了经快蓝标记且免疫标记的神经元轮廓的百分比。碳水化合物基团标记物的值分别为皮肤、肌肉和内脏的：凝集素花生凝集素为55%、24%和50%；凝集素大豆凝集素为72%、56%、61%；抗体2C5（针对乳糖系列基团）为43%、20%、6%；抗体SSEA - 4（针对球系列基团）为6%、12%、0%，抗体SSEA - 3（针对球系列基团）为6%、5%、0%。富含神经丝的轮廓的值分别为皮肤、肌肉和内脏的：34%、43%、19%，碳酸酐酶的值分别为10%、33%、2%。神经肽的值分别为降钙素基因相关肽为51%、70%、99%，P物质为21%、51%、82%，生长抑素为10%、2%、0%。因此，皮肤传入神经元群体中表达由2C5和凝集素标记的含半乳糖碳水化合物基团的轮廓比例最高，且生长抑素阳性细胞比例最高。相比之下，与其他组织相比，它们降钙素基因相关肽和P物质阳性细胞的比例最低。与其他组织相比，肌肉传入神经元中富含神经丝、碳酸酐酶阳性和SSEA - 4标记的轮廓比例最高，但凝集素结合的轮廓比例最低。与其他组织相比，内脏传入神经元中降钙素基因相关肽和P物质标记的神经元轮廓比例最高，但富含神经丝的轮廓以及碳酸酐酶或2C5标记的轮廓比例最低，并且它们完全缺乏球系列碳水化合物和生长抑素的任何标记。肌肉和皮肤传入神经元群体在其大小分布上都有明显的小细胞和大细胞峰值，皮肤传入神经元的小细胞峰值比肌肉传入神经元的大，肌肉传入神经元的大细胞峰值更明显。内脏传入神经元轮廓具有单峰大小分布，峰值大小介于躯体传入单位的小细胞和大细胞峰值之间。因此，这项研究表明，初级传入神经元的免疫组化标记模式和细胞大小根据其外周靶组织而有所不同。