[对在不同培养系统中获得的刚地弓形虫速殖子抗原的免疫化学研究]

[An immunochemical study of the antigens from Toxoplasma gondii tachyzoites obtained in different cultivation systems].

作者信息

Gladkova S E, Bormotov N I, Dedkova L M, Reshetnikov S S, Kurlaeva T B, Belanov E F

出版信息

Med Parazitol (Mosk). 1998 Jan-Mar(1):20-3.

Abstract

The protein composition and immunochemical properties of Toxoplasma gondii tachyzoites cultured on Vero cells and on mice were studied. Despite the fact that the main components of both preparations were shown to be proteins with molecular weights of 47, 34, 24, and 22 kDa, Toxoplasma-infected human sera antibodies interact mainly with the antigens of 66, 62, 57, 42, 38, 37, 36, 31, and 24 kDa. Comparing efficiency of enzyme immunoassay using the antigens of the tachyzoites obtained in different culture systems showed that the preparation of cultured Vero cells is similar to those of peritoneal exudates from infected mice and may be successfully used for the detection of antitoxoplasma antibodies in the sera of infected subjects.

摘要

对在Vero细胞和小鼠体内培养的刚地弓形虫速殖子的蛋白质组成和免疫化学特性进行了研究。尽管两种制剂的主要成分均显示为分子量为47、34、24和22 kDa的蛋白质，但弓形虫感染的人血清抗体主要与66、62、57、42、38、37、36、31和24 kDa的抗原相互作用。比较使用不同培养系统获得的速殖子抗原进行酶免疫测定的效率表明，Vero细胞培养制剂与感染小鼠的腹腔渗出液相似，可成功用于检测感染个体血清中的抗弓形虫抗体。