Dautu George, Ueno Akio, Miranda Aracelis, Mwanyumba Sophie, Munyaka Biscah, Carmen Gabriella, Kariya Tatsuya, Omata Yoshitaka, Saito Atsushi, Xuan Xuenan, Igarashi Makoto
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, 080-8555 Japan.
Exp Parasitol. 2008 Mar;118(3):362-71. doi: 10.1016/j.exppara.2007.09.010. Epub 2007 Oct 1.
We developed a sandwich ELISA for the detection of circulating Toxoplasma gondii MIC10 antigens. In T. gondii culture supernatant, MIC10 was detected in a growth dependent manner. Mice were infected with a lethal dose of either a virulent RH strain, an avirulent Beverley strain or a sub-lethal dose of a PLK strain of T. gondii. MIC10 appeared 2 days after infection and increased gradually in the sera of RH-infected mice. A detectable but significantly lower amount of MIC10 was observed in the sera of mice infected intraperitoneally with Beverley tachyzoites. In contrast, the MIC10 antigen in mice sera following oral infection with Beverley cysts was below detectable levels during the course of the experiment. In sera of PLK-infected mice, MIC10 was predominantly observed between late acute and early chronic phase. Our data show that the kinetics of circulating MIC10 differs depending on the strain and route of infection.
我们开发了一种夹心酶联免疫吸附测定法（ELISA）用于检测循环中的刚地弓形虫MIC10抗原。在刚地弓形虫培养上清液中，MIC10以生长依赖的方式被检测到。用致死剂量的强毒株RH、无毒株贝弗利或亚致死剂量的刚地弓形虫PLK株感染小鼠。感染后2天在RH感染小鼠的血清中出现MIC10，并逐渐增加。在腹腔感染贝弗利速殖子的小鼠血清中观察到可检测但显著较低量的MIC10。相反，在实验过程中，经口感染贝弗利包囊的小鼠血清中的MIC10抗原低于可检测水平。在PLK感染小鼠的血清中，MIC10主要在急性后期和慢性早期被观察到。我们的数据表明，循环MIC10的动力学因毒株和感染途径而异。
