Oda S, Kinoshita A, Higuchi T, Shizuya T, Ishikawa I
Department of Periodontology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
J Med Dent Sci. 1997 Sep;44(3):53-62.
We investigated ectopic bone formation by biphasic calcium phosphate (BCP) combined with recombinant human bone morphogenetic protein-2 (rhBMP-2) in the rat dorsum. Under reduced pressure, rhBMP-2 was adsorbed onto BCP, which consisted of 80% beta-tricalcium phosphate and 20% hydroxyapatite uniformly distributed in granules. Twenty Wistar rats were separated into 4 groups consisting of 5 animals each dosed with 2, 10, and 50 micrograms/700 microliters of rhBMP-2 and a control group (BCP only). Pieces of the BCP-BMP complex or only BCP were implanted under the dorsal skin of the rats. Histological sections were examined three weeks later. New bone was formed in all rats given 50 micrograms doses, but not in the 2 micrograms and control groups. These results indicated that BCP combined with rhBMP-2 induced ectopic bone formation without additional carriers. Therefore, BCP granules alone can function as carriers for rhBMP-2 to induce bone formation.
我们研究了双相磷酸钙（BCP）与重组人骨形态发生蛋白-2（rhBMP-2）联合在大鼠背部诱导异位骨形成的情况。在减压条件下，rhBMP-2被吸附到BCP上，BCP由80%的β-磷酸三钙和20%均匀分布于颗粒中的羟基磷灰石组成。将20只Wistar大鼠分为4组，每组5只，分别给予2、10和50微克/700微升的rhBMP-2，另设一个对照组（仅给予BCP）。将BCP-BMP复合物片或仅BCP植入大鼠背部皮肤下。三周后检查组织学切片。给予50微克剂量的所有大鼠均形成了新骨，但给予2微克剂量的大鼠和对照组未形成新骨。这些结果表明，BCP与rhBMP-2联合可在无额外载体的情况下诱导异位骨形成。因此，仅BCP颗粒就可作为rhBMP-2诱导骨形成的载体。
