Altiokka G, Tunçel M
Department of Analytical Chemistry, Faculty of Pharmacy, University of Anadolu, Eskişehir, Turkey.
J Pharm Biomed Anal. 1998 May;17(1):169-75. doi: 10.1016/s0731-7085(97)00211-2.
The optimum conditions using DC polarography and the determination of doxazosin employing SIAP and SCAP polarographic techniques are described in this study. All the experiments were conducted in the supporting electrolyte consisting of 20% ethanol (v/v), 0.2 M KCl and 0.2 M acetate buffer at various pH values in order to examine the optimum conditions, and pH 3.5 for the determination of doxazosin. Well-defined curves were obtained in the pH range of 1.5-7.5. The system was diffusional and irreversible at pH 3.5. The calibration studies were performed by using SIAP and SCAP polarography and satisfactory results were observed for all techniques. Since the sensitivity of SIAP and SCAP techniques were higher than the others, the determination of doxazosin was performed in filtered and unfiltered tablet solutions containing 4 mg active material. In the analysis of a tablet, the relative standard deviations (Srel %) of the techniques are in the filtered solutions +/- 0.9 (SIAP), +/- 0.8 (SCAP) and in the unfiltered solutions +/- 0.7 (SIAP), +/- 0.8 (SCAP) and no interference was observed during the analysis. The determination methods proposed in this study appear to be accurate, rapid and practicable. Therefore, these techniques may be suitable for the content uniformity tests.
本研究描述了使用直流极谱法的最佳条件以及采用单扫描极谱法（SIAP）和示差脉冲极谱法（SCAP）测定多沙唑嗪的方法。所有实验均在由20%乙醇（体积/体积）、0.2 M氯化钾和0.2 M醋酸盐缓冲液组成的支持电解质中进行，在不同pH值下以考察最佳条件，测定多沙唑嗪时的pH值为3.5。在1.5 - 7.5的pH范围内获得了清晰的曲线。该体系在pH 3.5时为扩散控制且不可逆。通过使用SIAP和SCAP极谱法进行了校准研究，所有技术均观察到了满意的结果。由于SIAP和SCAP技术的灵敏度高于其他技术，因此在含有4 mg活性物质的过滤和未过滤片剂溶液中进行了多沙唑嗪的测定。在片剂分析中，这些技术在过滤溶液中的相对标准偏差（Srel %）为±0.9（SIAP）、±0.8（SCAP），在未过滤溶液中为±0.7（SIAP）、±0.8（SCAP），且分析过程中未观察到干扰。本研究中提出的测定方法似乎准确、快速且实用。因此，这些技术可能适用于含量均匀度测试。
