Theodosiou N A, Xu T
Howard Hughes Medical Institute, Boyer Center for Molecular Medicine, Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06510, USA.
Methods. 1998 Apr;14(4):355-65. doi: 10.1006/meth.1998.0591.
Marked clones in mosaic animals have been used extensively to answer developmental questions in Drosophila. Recently, the use of the FLP/FRT system has allowed for the high-frequency production of mosaic animals for 95% of Drosophila genes. Cell markers have been engineered in this system to label mutant clones in both developing tissues and adult cuticle. Strains carrying these genetically marked FLP/FRT chromosomes have greatly enhanced our ability to study gene function in both germline and somatic Drosophila cells.
镶嵌动物中的标记克隆已被广泛用于解答果蝇发育方面的问题。最近，FLP/FRT系统的应用使得针对95%的果蝇基因高频产生镶嵌动物成为可能。在该系统中已构建了细胞标记，用于标记发育组织和成虫表皮中的突变克隆。携带这些遗传标记的FLP/FRT染色体的品系极大地增强了我们研究果蝇生殖细胞和体细胞中基因功能的能力。
