Benzinger E A, Emerick E, Grigsby N, Lovekamp M L, Logeman R, Sallee P J, Boster K L, Rehnstrom A, Riech A K, Small A T, Glasner J A, Coons T M, von Beroldingen C
Ohio Bureau of Criminal Identification and Investigation, London, USA.
J Forensic Sci. 1998 May;43(3):665-79.
A large number of reagents and steps are required for restriction fragment length polymorphism (RFLP) analysis, which at times make determining the cause of any observed anomaly difficult. Troubleshooting problems in RFLP analysis is difficult and often the exact cause of a problem cannot be determined. In this paper a collection of controlled experiments detail the consequences of a number of human or materials problems. Although the focus is on forensic applications, this troubleshooting guide will be helpful to anyone employing Southern analysis.
限制性片段长度多态性（RFLP）分析需要大量的试剂和步骤，这有时会使确定任何观察到的异常原因变得困难。对RFLP分析中的问题进行故障排除很困难，而且通常无法确定问题的确切原因。本文通过一系列对照实验详细阐述了一些人为或材料问题所产生的后果。尽管重点是法医应用，但本故障排除指南对任何采用Southern分析的人都会有所帮助。
