Strom C M, Rechitsky S
Department of Obstetrics and Gynecology, Illinois Masonic Medical Center, Chicago, USA.
J Forensic Sci. 1998 May;43(3):696-700.
Reliable single cell PCR requires nested or heminested PCR and careful optimization of conditions. This report describes the successful use of nested PCR for gender identification and reverse paternity testing in a forensic case where the only available materials consisted of charred human remains and a minute quantity of blood that were unsuitable for standard PCR. Use of nested PCR allowed the blood and burned tissue to be identified as human female. Analysis of two PCR length polymorphisms (AMPFLP) was successful on the blood sample and reverse paternity testing yielded a 98% probability that the blood spot was from the victim. The defendant was convicted of murder following a bench trial and the verdict was upheld by the Appellate court.
可靠的单细胞PCR需要巢式或半巢式PCR以及对条件进行仔细优化。本报告描述了在一个法医案件中成功使用巢式PCR进行性别鉴定和反向亲子鉴定的情况，在该案件中，唯一可用的材料是烧焦的人体遗骸和少量不适合标准PCR的血液。使用巢式PCR能够将血液和烧焦组织鉴定为人类女性。对血样成功进行了两种PCR长度多态性（AMPFLP）分析，反向亲子鉴定得出血斑来自受害者的概率为98%。在法官审讯后，被告被判犯有谋杀罪，上诉法院维持了原判。
