Ross P L, Davis P A, Belgrader P
DNA Technology Development Branch, Center for Medical and Molecular Genetics, Armed Forces Institute of Pathology, Rockville, Maryland 20850, USA.
Anal Chem. 1998 May 15;70(10):2067-73. doi: 10.1021/ac971256z.
Applications of polymerase chain reaction (PCR) product analysis using rapid affinity capture followed by delayed extraction (DE) MALDI-TOFMS is presented. Such applications include multiplex short tandem repeat (STR) typing, which is demonstrated for STR systems from conventional and microchip-based thermal cycling instruments. Using the combination of the microfabricated PCR instrument and DE-MALDI-TOFMS, a complete genotyping assay can be performed in under 50 min with a resultant molecular weight accuracy approaching or exceeding 100 ppm through external calibration. The observed resolution and mass accuracy for a 69-base PCR product enables identification of single base substitutions by direct molecular weight determination.
