[唾液腺肿瘤中DNA含量的测定]
[Determination of DNA content in salivary gland tumors].
作者信息
Yáñez M, Roa I, Villaseca M, Fuentealba P
机构信息
Unidad de Anatomía Patológica-Citopatología Hospital de Temuco, Chile.
出版信息
Rev Med Chil. 1997 Oct;125(10):1177-81.
BACKGROUND
DNA content determination is a useful tool in the characterization of different malignant tumors.
AIM
To measure DNA content in cells of salivary gland tumors as adjunct to histological diagnosis, correlating morphologic and biological features of these tumors.
MATERIAL AND METHODS
From the archives of the Pathology service of a general hospital, 21 salivary gland tumors, 15 pleomorphic adenomas, 3 mucoepidermoid carcinomas and 3 cystic adenoid carcinomas were selected. DNA content was determined in the histological samples using a flow cytometric DNA analysis.
RESULTS
All pleomorphic adenomas had a normal or diploid DNA content. Fifty percent of malignant tumors had an aneuploid DNA content (1 mucoepidermoid carcinoma and 2 cystic adenoid carcinomas).
CONCLUSIONS
DNA determination may help in the histological diagnosis of salivary gland tumors. The presence of aneuploidy suggests malignity.
背景
DNA含量测定是表征不同恶性肿瘤的一种有用工具。
目的
测量涎腺肿瘤细胞中的DNA含量，作为组织学诊断的辅助手段，并将这些肿瘤的形态学和生物学特征联系起来。
材料与方法
从一家综合医院病理科档案中选取21例涎腺肿瘤，其中15例多形性腺瘤、3例黏液表皮样癌和3例腺样囊性癌。采用流式细胞术DNA分析方法测定组织学样本中的DNA含量。
结果
所有多形性腺瘤的DNA含量均正常或为二倍体。50%的恶性肿瘤DNA含量为非整倍体（1例黏液表皮样癌和2例腺样囊性癌）。
结论
DNA测定可能有助于涎腺肿瘤的组织学诊断。非整倍体的存在提示恶性。
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