Horn T D, Haskell J
Division of Dermatopathology, The Johns Hopkins School of Medicine, Baltimore, Maryland, USA.
J Cutan Pathol. 1998 Apr;25(4):210-4. doi: 10.1111/j.1600-0560.1998.tb01721.x.
The lymphocytic subtypes effecting allogeneic graft-versus-host disease (GVHD) are unknown. We studied 35 skin biopsy specimens from 19 women transplanted with bone marrow from men for patterns and time course of infiltration of the skin by Y chromosome-bearing lymphocytes using in situ hybridization. Immunophenotypic analysis was performed on serial sections. Significant numbers of donor cells were first observed by day 13 after bone marrow transplantation (BMT), although a few cells were noted at earlier time points. The quantity of donor lymphocytes in the dermis correlated with the diagnosis of GVHD. For specimens with grade 1 features, only rare cells bore the Y chromosome, whereas the majority of lymphocytes in grade 2 tissues, whether heavily inflamed or not, contained the Y chromosome. These lymphocytes were predominantly CD4+ with fewer CD8+ and CD56+ cells in the dermis and epidermis. No concentration of a specific subtype in the epidermal compartment was observed. These data do not support the observation that a cutaneous graft-versus-host reaction (GVHR) is mediated primarily by CD8+ lymphocytes. Several effector cell populations may mediate a cutaneous GVHR with further variation over time and in BMTs between different individuals.
影响异基因移植物抗宿主病（GVHD）的淋巴细胞亚型尚不清楚。我们使用原位杂交技术，研究了19名接受男性骨髓移植的女性的35份皮肤活检标本，以观察含Y染色体淋巴细胞浸润皮肤的模式和时间进程。对连续切片进行免疫表型分析。骨髓移植（BMT）后第13天首次观察到大量供体细胞，不过在更早的时间点也发现了少数细胞。真皮中供体淋巴细胞的数量与GVHD的诊断相关。对于具有1级特征的标本，只有罕见细胞携带Y染色体，而2级组织中的大多数淋巴细胞，无论炎症程度如何，则都含有Y染色体。这些淋巴细胞主要为CD4 +，真皮和表皮中CD8 +和CD56 +细胞较少。未观察到表皮层中有特定亚型的聚集。这些数据不支持皮肤移植物抗宿主反应（GVHR）主要由CD8 +淋巴细胞介导的观点。几种效应细胞群体可能介导皮肤GVHR，且随着时间推移以及不同个体之间的骨髓移植情况而进一步变化。
