Inoue T, Fujito T, Hoshi K, Sakai Y, Morooka S, Sohma R
Department of Cardiology, Koshigaya Hospital, Dokkyo University School of Medicine, Koshigaya City, Saitama, Japan.
Thromb Haemost. 1998 May;79(5):1004-7.
Although platelet activation may play a role in coronary artery spasm, platelets activated following coronary vasospasm have not been clinically detected. We performed flow cytometric analysis of activation-dependent granular proteins, CD62P (P-selectin), CD63, PAC-1 (activated glycoprotein [GP] IIb/IIIa) and thrombospondin on the platelet plasma membrane in patients who exhibited acetylcholine-induced coronary vasospasm and compared findings with those in control patients without vasospasm. We simultaneously investigated the plasma levels of thrombin anti-thrombin III complex (TAT), plasmin alpha2-plasmin inhibitor complex (PIC), and thrombomodulin. In patients with vasospasm, the expression of CD62P, CD63 and PAC-1 on the platelet membrane surface increased in coronary sinus blood samples following coronary vasospasm, although the expression in aortic samples did not change. The TAT level also increased in the coronary sinus after vasospasm. Platelets might be activated by coronary vasospasm within the coronary circulation. The platelet activation process may be modulated by thrombin generation.
尽管血小板活化可能在冠状动脉痉挛中起作用，但冠状动脉痉挛后活化的血小板尚未在临床上被检测到。我们对表现出乙酰胆碱诱导的冠状动脉痉挛的患者血小板质膜上依赖活化的颗粒蛋白、CD62P（P选择素）、CD63、PAC-1（活化糖蛋白[GP]IIb/IIIa）和血小板反应蛋白进行了流式细胞术分析，并将结果与无痉挛的对照患者进行了比较。我们同时研究了血浆凝血酶抗凝血酶III复合物（TAT）、纤溶酶α2-纤溶酶抑制剂复合物（PIC）和血栓调节蛋白的水平。在痉挛患者中，冠状动脉痉挛后冠状窦血样中血小板膜表面CD62P、CD63和PAC-1的表达增加，尽管主动脉血样中的表达没有变化。痉挛后冠状窦中的TAT水平也升高。血小板可能在冠状动脉循环中被冠状动脉痉挛激活。血小板活化过程可能受凝血酶生成的调节。
