Sati H I, Apperley J F, Greaves M, Lawry J, Gooding R, Russell R G, Croucher P I
Department of Human Metabolism and Clinical Biochemistry, University of Sheffield Medical School.
Br J Haematol. 1998 May;101(2):287-95. doi: 10.1046/j.1365-2141.1998.00687.x.
Interleukin-6 (IL-6) is an important growth factor for human myeloma cells in vitro and in vivo. However, the identity of the cells producing IL-6 in vivo in patients with multiple myeloma (MM) remains the subject of debate. We have developed a sensitive dual-colour fluorescence in situ hybridization (FISH) technique to investigate the expression of IL-6 mRNA by individual bone marrow plasma cells from patients with multiple myeloma, monoclonal gammopathy of undetermined significance (MGUS) and healthy subjects. IL-6 mRNA could be identified in all immunoglobulin light chain (IgLC) expressing cells from all patients with MM and MGUS. The IL-6 protein could also be detected by direct immunofluorescence in all plasma cells (cytoplasmic light chain positive) from all patients with MM and MGUS. Furthermore, it was also possible to demonstrate cytoplasmic IL-6 staining of plasma cells from patients with MM by flow cytometric analysis. In contrast, neither the IL-6 mRNA or protein could be detected in normal plasma cells from healthy bone marrow donors. These data demonstrate that plasma cells from patients with MM and MGUS express the IL-6 mRNA and synthesize the IL-6 protein and support the hypothesis that autocrine synthesis of IL-6 is of importance in patients with MM.
白细胞介素-6（IL-6）在体外和体内都是人骨髓瘤细胞的重要生长因子。然而，多发性骨髓瘤（MM）患者体内产生IL-6的细胞身份仍存在争议。我们开发了一种灵敏的双色荧光原位杂交（FISH）技术，以研究来自多发性骨髓瘤患者、意义未明的单克隆丙种球蛋白病（MGUS）患者和健康受试者的单个骨髓浆细胞中IL-6 mRNA的表达。在所有MM和MGUS患者的所有表达免疫球蛋白轻链（IgLC）的细胞中都能鉴定出IL-6 mRNA。通过直接免疫荧光也能在所有MM和MGUS患者的所有浆细胞（细胞质轻链阳性）中检测到IL-6蛋白。此外，通过流式细胞术分析也有可能证明MM患者浆细胞的细胞质IL-6染色。相比之下，在健康骨髓供体的正常浆细胞中既检测不到IL-6 mRNA也检测不到IL-6蛋白。这些数据表明，MM和MGUS患者的浆细胞表达IL-6 mRNA并合成IL-6蛋白，支持IL-6自分泌合成在MM患者中具有重要意义这一假说。
