Sasaki K, Mori Y, Honda W, Miyake Y
Quality Control Department, Misato Plant, Eisai Company, Limited, Saitama, Japan.
PDA J Pharm Sci Technol. 1998 Mar-Apr;52(2):60-5.
For the purpose of selecting an appropriate biological indicator for evaluation of the effects of microwave heating sterilization, we examined aerobic bacterial spores to determine whether microwaves have non-thermal sterilization effects. After microwave irradiation on dry bacterial spores (three species), none of the bacterial spores were killed. The survival rate of the spores after microwave irradiation of spore suspensions (twelve species) was compared with that after heating by a conventional method. The order of heat resistance in the bacterial species was similar between the two heating methods. Bacillus stearothermophilus spores were the most heat-resistant. These results suggest that microwaves have no non-thermal sterilization effects on bacterial spores, the specific resistant spores to microwave heating, and microwave heating sterilization can be evaluated in the same way as for conventional heating sterilization. As a biological indicator for evaluation of overkill sterilization, B. stearothermophilus spores may be appropriate for microwave heating sterilization as well as steam sterilization.
为了选择合适的生物指示剂来评估微波加热灭菌的效果，我们检测了需氧芽孢杆菌，以确定微波是否具有非热灭菌效果。对干燥的芽孢杆菌孢子（三种）进行微波辐照后，没有一个芽孢被杀死。将芽孢悬浮液（十二种）微波辐照后的孢子存活率与传统方法加热后的存活率进行了比较。两种加热方法下细菌的耐热性顺序相似。嗜热脂肪芽孢杆菌孢子的耐热性最强。这些结果表明，微波对细菌孢子没有非热灭菌作用，细菌孢子对微波加热具有特定抗性，并且微波加热灭菌可以与传统加热灭菌以相同的方式进行评估。作为评估过度杀灭灭菌的生物指示剂，嗜热脂肪芽孢杆菌孢子可能适用于微波加热灭菌以及蒸汽灭菌。
