Udupi V, Townsend C M, Greeley G H
Department of Surgery, University of Texas Medical Branch, Galveston 77555, USA.
Biochem Biophys Res Commun. 1998 May 19;246(2):463-5. doi: 10.1006/bbrc.1998.8643.
The purpose of this study was to investigate the effects of activation of various second messenger signaling systems on gene expression (i.e. mRNA levels) of a peptide hormone processing enzyme called prohormone convertase-1 (PC-1, also called PC-3) in a human pancreatic carcinoid cell line (BON) that expresses several endocrine peptides (chromogranin A, pancreastatin, neurotensin). Pharmacologic activation of adenylate cyclase-cAMP, protein kinase-C and Ca2+ mobilization pathways increased PC-1 mRNA levels and neurotensin secretion. Elevations in PC-1 mRNA levels were dose and time-related. Secretagogue-induced cellular depletion of neurotensin was followed by a replenishment of cellular neurotensin stores and an upregulation of PC-1 mRNA levels. Together, these data indicate that PC-1 mRNA expression is increased with peptide secretion and coordinated with maintenance of cellular stores of peptides.
本研究的目的是在表达几种内分泌肽（嗜铬粒蛋白A、胰抑制素、神经降压素）的人胰腺类癌细胞系（BON）中，研究激活各种第二信使信号系统对一种名为激素原转化酶-1（PC-1，也称为PC-3）的肽激素加工酶的基因表达（即mRNA水平）的影响。腺苷酸环化酶-cAMP、蛋白激酶-C和Ca2+动员途径的药理学激活增加了PC-1 mRNA水平和神经降压素分泌。PC-1 mRNA水平的升高与剂量和时间相关。促分泌剂诱导的细胞内神经降压素耗竭之后是细胞内神经降压素储存的补充和PC-1 mRNA水平的上调。总之，这些数据表明，PC-1 mRNA表达随着肽分泌增加，并与细胞内肽储存的维持相协调。
