Li D, Ong C N, Ng V, Tan C
Department of Environmental Health, Sun Yat-sen University of Medical Sciences, Guangzhou, PR China.
Vet Hum Toxicol. 1998 Jun;40(3):129-31.
The study was carried out to observe the effect of nickel chloride (NiCl2) on T-lymphocyte subsets, CD4, CD8 and CD4/CD8 ratios in vivo. BALB/C mice were injected i.p. with NiCl2 every other day for 2 w. The NiCl2 doses were 1, 3, 6, 9 or 12 mg/kg. Lymphocytes were obtained from mouse spleen and cultured in the presence of monoclonal antibody FITC conjugated, anti-CD4 or anti-CD8. Samples were determined with a flow cytometer. A suppressive effect on CD4-positive cells was found in the 6 mg/kg group, but this subset was restored to the control level in the 9 and 12 mg/kg groups, suggesting that there was an "immune tolerance" effect. No significant changes were found in CD8-positive cells and CD4/CD8 ratios. The results suggested the NiCl2 had a deleterious effect on mouse T-lymphocytes in short-term in vivo exposure, but the effects may depend on the dose and cell subset.
本研究旨在观察氯化镍（NiCl₂）对体内T淋巴细胞亚群、CD4、CD8及CD4/CD8比值的影响。每隔一天给BALB/C小鼠腹腔注射NiCl₂，持续2周。NiCl₂剂量分别为1、3、6、9或12 mg/kg。从小鼠脾脏获取淋巴细胞，并在结合有荧光素异硫氰酸酯（FITC）的抗CD4或抗CD8单克隆抗体存在的情况下进行培养。用流式细胞仪测定样本。在6 mg/kg组中发现对CD4阳性细胞有抑制作用，但在9和12 mg/kg组中该亚群恢复到对照水平，提示存在“免疫耐受”效应。CD8阳性细胞及CD4/CD8比值未发现明显变化。结果表明，短期体内暴露时NiCl₂对小鼠T淋巴细胞有有害作用，但其影响可能取决于剂量和细胞亚群。
