Hancock R J
Queen's Hospital, Burton Hospitals National Health Service Trust, Burton-on-Trent, Staffordshire, England.
Urology. 1998 May;51(5A Suppl):146-9. doi: 10.1016/s0090-4295(98)00075-2.
a) To assess the use of "panning" procedures for the detection of antibodies to sperm. b) To develop and use human monoclonal antibodies for the analysis of human transplantation antigens.
"Panning" assays were developed. The procedures and their use in the analysis of mouse monoclonal antibodies to human sperm are described. B-lymphocytes from individuals sensitized by pregnancy, graft rejection, or blood transfusion were immortalized with Epstein-Barr virus (EBV). Selected sublines producing antibodies to human leukocyte series A (HLA) antigens were fused with mouse myeloma cells and selected clones of hybrid cells secreting monoclonal antibodies were selected.
The results of panning assays correlated with those of agglutination assays, but not immunofluorescence or enzyme-linked immunosorbent assays (ELISA). Monospecific and cross-reactive human monoclonal antibodies to HLA antigens were produced.
Panning assays can be used for the detection of antibodies to sperm and can be useful where target sperm are of poor motility. The assays may be more sensitive for antibodies to superficial antigens. Human monoclonal antibodies may be used for investigating the identity of, and responses to, transplantation antigens.
a) 评估“淘选”程序在检测抗精子抗体中的应用。b) 开发并使用人单克隆抗体分析人类移植抗原。
开发了“淘选”试验。描述了这些程序及其在分析小鼠抗人精子单克隆抗体中的应用。通过妊娠、移植排斥或输血致敏个体的B淋巴细胞用爱泼斯坦-巴尔病毒（EBV）永生化。将产生抗人白细胞A系列（HLA）抗原抗体的选定亚系与小鼠骨髓瘤细胞融合，并筛选分泌单克隆抗体的杂交细胞克隆。
淘选试验结果与凝集试验结果相关，但与免疫荧光或酶联免疫吸附试验（ELISA）无关。产生了针对HLA抗原的单特异性和交叉反应性人单克隆抗体。
淘选试验可用于检测抗精子抗体，在目标精子活力差的情况下可能有用。该试验对表面抗原抗体可能更敏感。人单克隆抗体可用于研究移植抗原的特性及对其的反应。
