van der Weiden R M, Helmerhorst F M, Keirse M J
Department of Obstetrics, Gynaecology and Reproduction, Leiden University Hospital, The Netherlands.
Prostaglandins Leukot Essent Fatty Acids. 1998 Mar;58(3):205-7. doi: 10.1016/s0952-3278(98)90115-6.
The non-enzymatic metabolites of prostacyclin (PGI2) and thromboxane A2 (TXA2), 6-keto-prostaglandin F1alpha (6-keto-PGF1alpha) and thromboxane B2 (TXB2), and their 2,3-dinor metabolites, 2,3-dinor-6-keto-PGF1alpha and 2,3-dinor-TXB2, were measured in early morning urine samples in 24 in vitro fertilization (IVF) cycles in 24 women and in 27 women who became pregnant after IVF and embryo transfer (ET). The sum of the non-enzymatic metabolites and their 2,3-dinor metabolites was considered to be a reflection of total PGI2 and total TXA2 production in vivo. Both the ratio of 'total' PGI2/'total' TXA2 and the ratio of the 2,3-dinor metabolites were calculated. TXB2 concentrations showed virtually no change and the ratios of the non-enzymatic metabolites of PGI2 and TXA2 versus their 2,3-dinor metabolites remained relatively constant. As a consequence, the ratio of 2,3-dinor-6-keto-PGF1alpha/2,3-dinor-TXB2 was a close reflection of the ratio of 'total' PGI2/'total' TXA2, although the latter ratio was significantly higher all the time. We conclude that for comparative studies on the balance between PGI2 and TXA2 in IVF cycles and during gestation, the determination of the 2,3-dinor metabolites alone can replace the measurement of all four metabolites.
在24名接受体外受精（IVF）的女性以及27名在IVF和胚胎移植（ET）后怀孕的女性清晨尿液样本中，检测了前列环素（PGI2）和血栓素A2（TXA2）的非酶代谢产物6-酮-前列腺素F1α（6-酮-PGF1α）和血栓素B2（TXB2），以及它们的2,3-二去甲代谢产物2,3-二去甲-6-酮-PGF1α和2,3-二去甲-TXB2。非酶代谢产物及其2,3-二去甲代谢产物的总和被认为反映了体内PGI2和TXA2的总生成量。计算了“总”PGI2/“总”TXA2的比值以及2,3-二去甲代谢产物的比值。TXB2浓度几乎没有变化，PGI2和TXA2的非酶代谢产物与其2,3-二去甲代谢产物的比值保持相对恒定。因此，2,3-二去甲-6-酮-PGF1α/2,3-二去甲-TXB2的比值密切反映了“总”PGI2/“总”TXA2的比值，尽管后者的比值始终显著更高。我们得出结论，对于IVF周期和妊娠期PGI2与TXA2平衡的比较研究，仅测定2,3-二去甲代谢产物就可以替代对所有四种代谢产物的测量。
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