Bagyan I, Noback M, Bron S, Paidhungat M, Setlow P
Department of Biochemistry, University of Connecticut Health Center, Farmington, CT 06032, USA.
Gene. 1998 Jun 8;212(2):179-88. doi: 10.1016/s0378-1119(98)00172-3.
A new Bacillus subtilis sporulation-specific gene, yhcN, has been identified, the expression of which is dependent on the forespore-specific sigma factor sigmaG and to a much lesser extent on sigmaF. A translational yhcN-lacZ fusion is expressed at a very high level in the forespore, and the protein encoded by yhcN was detected in the inner spore membrane. A yhcN mutant sporulates normally and yhcN spores have identical resistance properties to wild-type spores. However, the outgrowth of yhcN spores is slower than that of wild-type spores.
一个新的枯草芽孢杆菌芽孢形成特异性基因yhcN已被鉴定出来，其表达依赖于前芽孢特异性的σ因子σG，且在较小程度上依赖于σF。一个yhcN-lacZ翻译融合体在前芽孢中以非常高的水平表达，并且在芽孢内膜中检测到了yhcN编码的蛋白质。yhcN突变体正常形成芽孢，yhcN芽孢与野生型芽孢具有相同的抗性特性。然而，yhcN芽孢的萌发比野生型芽孢慢。
