在特定培养基中对淋病奈瑟菌和脑膜炎奈瑟菌产生的一些基本可提取化合物的鉴定。
Identification of some basic extractable compounds produced by Neisseria gonorrhoeae and Neisseria meningitidis in a defined medium.
作者信息
Brooks J B, Kellogg D S, Choudhary G, Alley C C, Liddle J A
出版信息
J Clin Microbiol. 1978 May;7(5):415-8. doi: 10.1128/jcm.7.5.415-418.1978.
Abstract
Electron capture gas-liquid chromatography and mass spectrometry have been used to identify some of the basic extractable heptafluorobutyric anhydride reactive compounds found in a defined medium after 20 h of growth by Neisseria gonorrhoeae and N. meningitidis. Acetoin, 2,3-butanediol, pyrroline, and 1,3-diaminopropane were identified by both gas chromatography and mass spectrometry; 2-hydroxy-pyrrolidine and 3-aminomethyl-pyrrolidine were tentatively identified by mass spectrometry. A possible origin of the amines is through enzymatic oxidation of polyamines.
摘要
采用电子捕获气液色谱法和质谱法，对淋病奈瑟菌和脑膜炎奈瑟菌在特定培养基中生长20小时后发现的一些可提取的七氟丁酸酐反应性碱性化合物进行了鉴定。通过气相色谱法和质谱法鉴定出了乙偶姻、2,3-丁二醇、吡咯啉和1,3-二氨基丙烷；通过质谱法初步鉴定出了2-羟基吡咯烷和3-氨基甲基吡咯烷。胺类物质的一个可能来源是多胺的酶促氧化。
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本文引用的文献
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The oxidation of polyamines by Neisseria perflava.全黄奈瑟菌对多胺的氧化作用。
J Biol Chem. 1958 Apr;231(2):647-55.
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Electron capture gas chromatographic detection of acethylmethylcarbinol produced by neisseria gonorrhoeae.电子捕获气相色谱法检测淋病奈瑟菌产生的乙酰甲基甲醇。
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3
Identification of Neisseria by electron capture gas-liquid chromatography of metabolites in a chemically defined growth medium.在化学成分明确的生长培养基中，通过代谢产物的电子捕获气液色谱法鉴定奈瑟菌。
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