[Determination and identification of beta-1,3;1,6-glycooligosaccharides in laminarioligosaccharide mixtures by means of enzymologic transglycosylation. Oligosaccharides with degree of polymerization of 5 and 6].

1-->3; 1-->6-beta-D-Glucooligosaccharides with polymerization degree of 5-6 were obtained from laminaran and analyzed by the previously developed method [1] by using them as donors and p-nitrophenyl beta-D-glucoside as the acceptor in the transglycosylation reaction catalysed by endo-1-->3-beta-D-glucanase LIV. The resulting homologous p-nitrophenyl beta-1,3-laminarioligosides with polymerization degree of 2-6 and the corresponding derivatives of mixed beta-1,3; 1,6-glucooligosaccharides with the same polymerization degree were analyzed by HPLC. The latter compounds exhibited higher retention times than the former with the same polymerization degree. Isomeric tetra-, penta-, and hexameric compounds were detected, and some of them were structurally characterized by means of NMR. The suggested method of analysis of oligosaccharide mixtures is simple, informative, and consumes a minimal quantity of sample.