Naumov G I, Naumova E S, Marinoni G, Piskur J
State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russia.
Genetika. 1998 Apr;34(4):565-8.
Genetic lines of three biological yeast species of the Saccharomyces sensu lato group--S. castellii, S. exiguus, and S. martiniae--were created. Homo- and heterothalmic monosporic fertile strains were marked by different auxotrophic mutations. Conditions for the hybridization of the Saccharomyces sensu lato yeast were established. The mating of spores or haploid cells of auxotrophic complementary mutants was performed on the complete YPD medium. Prototrophic diploid hybrids were selected on a minimal medium. In S. castellii, the hybridization of diploid cells was demonstrated. All three species studied were suitable for tetrad analysis.
构建了酿酒酵母广义组的三种生物学酵母物种——卡氏酵母、嗜杀酵母和马丁酵母的遗传株系。同宗和异宗单孢子可育菌株通过不同的营养缺陷型突变进行标记。确定了广义酿酒酵母的杂交条件。营养缺陷型互补突变体的孢子或单倍体细胞在完全YPD培养基上进行交配。在基本培养基上选择原养型二倍体杂种。在卡氏酵母中，证明了二倍体细胞的杂交。所研究的所有三个物种都适用于四分体分析。
