Cavallo J D, Hernandez E, Gerome P, Plotton N, Debord T, Le Vagueresse R
Laboratoire de Biologie, l'Hôpital d'Instruction des Armées Bégin, Saint Mandé, France.
Med Trop (Mars). 1997;57(4):353-6.
Serum levels of HRP-2 antigens against Plasmodium falciparum in 568 venous blood samples collected at two general hospitals were evaluated using the ParaSight-F (Becton-Dickinson) alone (568/568 samples) or in combination (156/568 samples) with the new ICT-Malaria P.f. (ICT-diagnostic). Comparison with the reference method (thin and thick blood smears assessed by two experienced parasitologists) showed that both tests were highly sensitive (93% and 96% respectively) and specific (98% and 98% respectively). The positive predictive values of the two tests were equal (96%) and the negative predicative values were close (96% and 98% respectively). Although both tests provided results within 10 minutes and required no special equipment for interpretation, the ICT-Malaria P.f. test seemed simpler and easier to use than the ParaSight-F test. The ParaSight-F test alone was used to monitor serum antigen levels after treatment in 24 patients. Antigen levels remained positive for at least three days after disappearance of circulating parasites (range: 3 to 28 days). Evaluation of serum levels of HRP-2 antigens can be useful for emergency diagnosis, especially in patients with low circulating parasite levels. The ICT-Malaria P.f. test seems especially suited for in-field use.
在两家综合医院采集的568份静脉血样本中，单独使用帕拉西特-F（百特公司生产）（568/568份样本）或与新型ICT-恶性疟原虫检测法（ICT诊断法）联合使用（156/568份样本），对恶性疟原虫HRP-2抗原的血清水平进行了评估。与参考方法（由两名经验丰富的寄生虫学家评估薄血膜和厚血膜）相比，两种检测方法均具有高灵敏度（分别为93%和96%）和高特异性（分别为98%和98%）。两种检测方法的阳性预测值相等（96%），阴性预测值相近（分别为96%和98%）。虽然两种检测方法都能在10分钟内得出结果，且无需特殊设备进行判读，但ICT-恶性疟原虫检测法似乎比帕拉西特-F检测法更简单易用。单独使用帕拉西特-F检测法对24例患者治疗后的血清抗原水平进行了监测。循环中的疟原虫消失后，抗原水平至少持续三天呈阳性（范围：3至28天）。评估HRP-2抗原的血清水平对急诊诊断可能有用，尤其是对于循环疟原虫水平较低的患者。ICT-恶性疟原虫检测法似乎特别适合现场使用。
