German A J, Bland P W, Hall E J, Day M J
Department of Clinical Veterinary Science, University of Bristol, Langford, UK.
Vet Immunol Immunopathol. 1998 Feb 27;61(2-4):171-80. doi: 10.1016/s0165-2427(97)00144-x.
Immunohistochemical techniques were used to assess major histocompatibility complex (MHC) class II expression by enterocytes and lamina propria cells in the canine intestinal tract. Duodenal enterocyte class II expression was faint and limited to the lower crypt region whereas jejunal and ileal enterocyte expression was stronger, being present in both crypt and villus areas. Enterocyte staining was of greatest intensity in crypts adjacent to Peyer's patches and intense membrane staining of most Peyer's patch lymphocytes was also seen. Enterocyte MHC class II expression in the colon was largely limited to the lower crypt region. Within the lamina propria, of all intestinal sites examined, a heterogeneous population of cells were MHC class II positive and these had morphological features of macrophages and dendritic cells. Lymphocytes, plasma cells, fibroblasts and vascular endothelium were not stained. Definition of constitutive expression of MHC class II within the canine intestine may be important in identifying upregulation of this molecule in inflammatory bowel diseases.
采用免疫组织化学技术评估犬肠道中肠上皮细胞和固有层细胞的主要组织相容性复合体（MHC）II类表达。十二指肠肠上皮细胞II类表达微弱，局限于隐窝下部区域，而空肠和回肠肠上皮细胞表达较强，在隐窝和绒毛区域均有表达。在与派尔集合淋巴结相邻的隐窝中，肠上皮细胞染色强度最大，并且还可见大多数派尔集合淋巴结淋巴细胞的强烈膜染色。结肠中的肠上皮细胞MHC II类表达主要局限于隐窝下部区域。在所有检查的肠道部位的固有层内，有一群异质性细胞为MHC II类阳性，这些细胞具有巨噬细胞和树突状细胞的形态特征。淋巴细胞、浆细胞、成纤维细胞和血管内皮均未染色。确定犬肠道内MHC II类的组成性表达对于识别炎症性肠病中该分子的上调可能很重要。
