Jacob H J, Christ B, Jacob M, Ahlström P
Acta Anat (Basel). 1976;94(2):204-20.
In order to study the possible influence of adjacent structures upon differentiation and proliferation of the chick spinal cord, a piece of neural tube of chick embryos incubated for 2 days (stages 12-14 of HAMBURGER and HAMILTON) was extirpated and replaced by an equal graft from a donor embryo of the same age. The grafts were rotated 90 or 180 degrees around their longitudinal axis. The examination of the serial histological sections and the analysis of the distribution of mitotic density in the rotated spinal cord of embryos fixed 2-8 days after the operation led to the following results: the differences in morphogenesis and histogenesis between dorsal and ventral sectors of the spinal cord are already determined in 2-day-old chick embryos. The reversal of the neural tube at this time has no effect either upon the typical differentiation or the characteristic distribution of the mitotic cells in the spinal cord.
为了研究相邻结构对鸡脊髓分化和增殖的可能影响，切除了孵化2天（汉伯格和汉密尔顿第12 - 14阶段）的鸡胚的一段神经管，并用来自相同年龄供体胚胎的等量移植物进行替换。移植物绕其纵轴旋转90度或180度。对术后2 - 8天固定的胚胎旋转脊髓的连续组织学切片检查和有丝分裂密度分布分析得出以下结果：脊髓背侧和腹侧部分在形态发生和组织发生上的差异在2日龄鸡胚中就已确定。此时神经管的反转对脊髓中典型分化或有丝分裂细胞的特征分布均无影响。
