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利用合成肽研究碱性成纤维细胞生长因子及其受体FGFR-1的假定肝素结合结构域与肝素的相互作用。

Interactions of putative heparin-binding domains of basic fibroblast growth factor and its receptor, FGFR-1, with heparin using synthetic peptides.

作者信息

Kinsella L, Chen H L, Smith J A, Rudland P S, Fernig D G

机构信息

Cancer Laboratory, School of Biological Sciences, Liverpool, UK.

出版信息

Glycoconj J. 1998 Apr;15(4):419-22. doi: 10.1023/a:1006986104865.

DOI:10.1023/a:1006986104865
PMID:9613830
Abstract

We have examined structure-function relationships that have been proposed to account for the heparin-binding properties of basic fibroblast growth factor and its receptor, FGFR-1, using synthetic peptides, DNA synthesis assays and binding assays in a resonant mirror biosensor. The results suggest that the interaction of FGFR-1 with heparin may not be physiologically relevant and that the site of interaction of the polysaccharide on bFGF is more complex than has been anticipated.

摘要

我们使用合成肽、DNA合成测定法以及共振镜生物传感器中的结合测定法，研究了为解释碱性成纤维细胞生长因子及其受体FGFR-1的肝素结合特性所提出的结构-功能关系。结果表明，FGFR-1与肝素的相互作用可能与生理无关，并且多糖在bFGF上的相互作用位点比预期的更为复杂。

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Interactions of putative heparin-binding domains of basic fibroblast growth factor and its receptor, FGFR-1, with heparin using synthetic peptides.利用合成肽研究碱性成纤维细胞生长因子及其受体FGFR-1的假定肝素结合结构域与肝素的相互作用。
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Kinetics of protein-protein interactions at the surface of an optical biosensor.光学生物传感器表面蛋白质 - 蛋白质相互作用的动力学
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Fibroblast growth factors and their receptors: an information network controlling tissue growth, morphogenesis and repair.成纤维细胞生长因子及其受体：一个控制组织生长、形态发生和修复的信息网络。
Prog Growth Factor Res. 1994;5(4):353-77. doi: 10.1016/0955-2235(94)00007-8.
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Endothelial heparan sulfate proteoglycans that bind to L-selectin have glucosamine residues with unsubstituted amino groups.与L-选择素结合的内皮硫酸乙酰肝素蛋白聚糖含有带有未取代氨基的葡糖胺残基。
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Rat mammary preadipocytes in culture produce a trophic agent for mammary epithelia-prostaglandin E2.培养中的大鼠乳腺前脂肪细胞可产生一种针对乳腺上皮细胞的营养因子——前列腺素E2。
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