Olver S D, Price P
Department of Microbiology, University of Western Australia, Nedlands, Australia.
Int J Exp Pathol. 1998 Feb;79(1):33-46.
We characterized liver-infiltrating leucocytes (LIL) from BALB/c and C57BL/6 mice 0-56 days after murine cytomegalovirus (MCMV) infection. Inflammation clears from C57BL/6 mice 4-5 weeks post infection (pi), but persists for several months in BALB/c mice. The LIL obtained were 60-80% Thy 1.2+ by flow cytometry. The percentage of CD8+ cells rose sharply in all mice 7 days pi, with little decrease in BALB/c mice by day 56. CD4-CD8-Thy 1.2+/TCR alpha beta + cells were more prevalent in LIL than lymph node cells (LNC) irrespective of MCMV infection, whilst infection increased the proportion of CD8+ L-selectin- LIL (but not LNC). LIL from both mouse strains demonstrated cytotoxic activity against YAC-1 cells, but only LIL from BALB/c mice proliferated spontaneously ex vivo 21 days pi, as measured by tritiated thymidine incorporation. BALB/c LIL produced IFN gamma and IgG2a 7-21 days pi, whilst IL-10 secretion was similar in both strains. Thus, persistent hepatitis in BALB/c mice is associated with activation and proliferation of intrahepatic leucocytes with some bias towards a Th1 response.
我们对感染鼠巨细胞病毒（MCMV）后0至56天的BALB/c和C57BL/6小鼠的肝脏浸润白细胞（LIL）进行了特征分析。感染后4至5周，炎症在C57BL/6小鼠中清除，但在BALB/c小鼠中持续数月。通过流式细胞术检测，所获得的LIL中有60%至80%为Thy 1.2+。感染后7天，所有小鼠体内CD8+细胞的百分比急剧上升，到第56天时，BALB/c小鼠体内的CD8+细胞百分比几乎没有下降。无论是否感染MCMV，CD4-CD8-Thy 1.2+/TCRαβ+细胞在LIL中比在淋巴结细胞（LNC）中更为普遍，而感染增加了CD8+ L-选择素-LIL（而非LNC）的比例。两种小鼠品系的LIL均对YAC-1细胞表现出细胞毒性活性，但只有BALB/c小鼠的LIL在感染后21天能在体外自发增殖，这通过氚化胸腺嘧啶核苷掺入法进行测定。BALB/c LIL在感染后7至21天产生IFNγ和IgG2a，而两种品系中IL-10的分泌相似。因此，BALB/c小鼠中持续性肝炎与肝内白细胞的激活和增殖有关，且存在一定的Th1反应偏向。
