Takehisa J, Zekeng L, Ido E, Mboudjeka I, Moriyama H, Miura T, Yamashita M, Gürtler L G, Hayami M, Kaptué L
Laboratory of Viral Pathogenesis, Kyoto University, Japan.
Virology. 1998 May 25;245(1):1-10. doi: 10.1006/viro.1998.9141.
In order to assess the incidence of HIV mixed infection as well as to clarify the molecular epidemiology of HIV in central Africa, we investigated 43 HIVs obtained from 211 Cameroonian AC, ARC, and AIDS patients in 1994 and 1995. Part of the pol region and part of the env region were phylogenetically analyzed. The genotypes observed were varied: of 43 specimens, 28 (65%) were subtype A, 1 (2%) was subtype B, 2 (5%) were subtype D, 3 (7%) were subtype F, and 2 (5%) were group O. Of the remaining 7 specimens, 3 were mixed infections with HIV-1 subtypes A and C, HIV-1 subtypes C and F, and HIV-2 subtype A and HIV-1 subtype A; 1 was a mixed infection with HIV-1 subtypes A and D and the highly divergent group O (triple infection); another 3 appeared to consist of mosaic genomes (A/G, A/E, and B/A recombinant). These data show that various types of mixed infection, such as between different subtypes of HIV-1 group M, between HIV-1 and HIV-2, and even between HIV-1 groups O and M, were confirmed at a rather high frequency (approximately 10%). The mixed infection is particularly significant where there is a greater variety of HIV-1 subtypes circulating, since it results in new genetic diversity generated by intersubtype recombination.
为了评估艾滋病毒混合感染的发生率以及阐明中非地区艾滋病毒的分子流行病学，我们调查了1994年和1995年从211名喀麦隆艾滋病相关综合征（AC）、艾滋病相关症（ARC）和艾滋病患者身上获取的43株艾滋病毒。对部分pol区域和部分env区域进行了系统发育分析。观察到的基因型各不相同：在43个样本中，28个（65%）为A亚型，1个（2%）为B亚型，2个（5%）为D亚型，3个（7%）为F亚型，2个（5%）为O组。其余7个样本中，3个是艾滋病毒1型A和C亚型、艾滋病毒1型C和F亚型以及艾滋病毒2型A和艾滋病毒1型A的混合感染；1个是艾滋病毒1型A和D亚型与高度分化的O组的混合感染（三重感染）；另外3个似乎由镶嵌基因组组成（A/G、A/E和B/A重组体）。这些数据表明，不同类型的混合感染，如艾滋病毒1型M组不同亚型之间、艾滋病毒1型和艾滋病毒2型之间，甚至艾滋病毒1型O组和M组之间的混合感染，都以相当高的频率（约10%）得到证实。在有更多种艾滋病毒1型亚型传播的地方，混合感染尤为显著，因为它会导致由亚型间重组产生新的基因多样性。
