Khatri A, Both G W
CSIRO, North Ryde, New South Wales, Australia.
Virology. 1998 May 25;245(1):128-41. doi: 10.1006/viro.1998.9136.
The ovine adenovirus isolated OAV287 represents a new group of adenoviruses that are distinct from the Mast- and Aviadenoviruses by several criteria, including genome arrangement. The OAV major late promoter and some late transcripts were previously mapped. To better define the probable coding sequences and to identify the approximate location of early promoters a partial transcription map of the genome was elucidated using a PCR-based approach. This was possible because the complete nucleotide sequence of the genome was known. The strategy permitted the identification of transcription start sites and RNA splice junctions and allowed the approximate location of promoters in the lefthand end, IVa2, E2, P32K, and E4 regions to be deduced. The data showed that lefthand end and E4 regions are controlled by three and two temporally distinct promoters, respectively. The E2 region is controlled by a single promoter, in contrast to Mastadenoviruses, where E2 expression is controlled by the E2A and E2B promoters. The p32kDa structural protein at the lefthand end and the IVa2 protein are also expressed from their own promoters. These data contribute to the first overview of transcription from a non-Mastadenovirus genome.
分离得到的绵羊腺病毒OAV287代表了一类新的腺病毒，根据包括基因组排列在内的几个标准，它与 Mast腺病毒和禽腺病毒不同。OAV主要晚期启动子和一些晚期转录本先前已被定位。为了更好地确定可能的编码序列并识别早期启动子的大致位置，使用基于PCR的方法阐明了基因组的部分转录图谱。这之所以可行，是因为基因组的完整核苷酸序列是已知的。该策略允许识别转录起始位点和RNA剪接连接点，并能推断出左手端、IVa2、E2、P32K和E4区域中启动子的大致位置。数据表明，左手端和E4区域分别由三个和两个时间上不同的启动子控制。与 Mast腺病毒不同，E2区域由单个启动子控制，在Mast腺病毒中，E2表达由E2A和E2B启动子控制。左手端的p32kDa结构蛋白和IVa2蛋白也从它们自己的启动子表达。这些数据有助于首次概述非Mast腺病毒基因组的转录情况。
